User:ShaoyingLu

Shaoying Lu's Weekly Schedule

Time	Sat	Sun	Mon	Tue	Wed	Thur	Fri
9am-12pm	-	-	Mechanobiological meeting and computational membrane meeting	-	-	-	-
1pm-5pm	-	-	-	-	-	-	-
6pm-10pm	-	-	-	-	-	-	-

Time	Sat	Sun	Mon	Tue	Wed	Thur	Fri
9am-12pm	-	Imaging NES-Ypet-Src biosensor in MEF cells	Talked to Jackie Zhou on edge detection	Pass cells	Transfection	Pass cells	Imaging Lyn-Src in MEF on fibronectin coated dish
1pm-5pm	-	-	-	-	-	-	-
6pm-10pm	-	-	-	-	-	-	-

Time	Sat	Sun	Mon	Tue	Wed	Thur	Fri
9am-12pm	transfection, prepare FN dishes	-	Failed imaging, low fluorescence	fix hard drive	Image analysis	learn from Jihye to amplify DNA	pass cells
1pm-5pm	-	pass cells	fix hard drive	Image analysis and read about linear regression	Image analysis	-	-
6pm-10pm	-	-	-	-	-	-	-

Time	Sat	Sun	Mon	Tue	Wed	Thur	Fri
9am-12pm	transfection, prepare FN dishes	-	autoclave vials; Imaging	Order Co2, Analysis	place order, transfection	pick up co2 tanks, pass cells, FE analysis	imaging Lyn-Src-YFP in MEF on fibronectin
1pm-5pm	-	pass cells	Imaging MEF cells with Lyn-YFP-Src	Analysis	make fibronectin dish, change medium	-	imaging
6pm-10pm	-	Paybills	Imaging	Analysis	-	-	imaging

Time	Sat	Sun	Mon	Tue	Wed (07/04)	Thur	Fri
9am-12pm	-	-	Organize Notes	Tansfection	Tansfection	Pass cells; Imaging Lyn-Src+PDGF in MEF cells	Imaging failed
1pm-5pm	-	-	Read Papers	Make CO2 independent medium	Prepare fibronectin coat	Imaging , prepare fibronectin coat	write up FEM
6pm-10pm	-	-	Read Papers	-	See fireworks	-	vedio conference

Time	Sat	Sun	Mon	Tue	Wed	Thur	Fri
9am-12pm	-	-	-	-	Imaging YFP-Cyto-Src Hela	-	-
1pm-5pm	-	-	Meet at lunch, plot alpha vs FRET ratio	Plot for two-compartment model	Imaging YFP-Lyn-Src of Hela	-	Labmeeting (1:30-4:30)
6pm-10pm	-	-	Mingxing's Data	-	Imaging	-	-