User:Shahar Sukenik/Notebook/p53CT expression in minimal media/2010/08/04

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04/08/10 Took 1.5 mL sample from ctrl flask, centrifuged at 4C, 12k RPM. Froze sup. and peller in Ronen's box at Tsafi's. In addition took an extra 10 mL of culture and froze in the fridge at Tsafi's. Since M9 showed no growth, cultures were thrown out. Next step: grow in MOPS minimal media - M9 must be too poor for this strain. MOPS prep: Prepared and froze 10x MOPS mixture. Autoclaved 0.5 L DDW, K2HPO4 and glassware for expression. booked incubator for 17°C for next monday. Note: There is still a large stock of everything needed for MOPS - all I will have to do is make new 15NHCl4 solution.