User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/06/25

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Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.

  • Waiting for sequencing results.

Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.

  • Isolated pBEST-SphI//NcoI-deGFP-T500 vector backbone digest product via gel extraction.
    • Gel eletrophoresis observation: expected 3096 bp and 106 bp dsDNA bands, observed . The larger band was the one purified from the gel.
  • I ligated the different linkers into the pBEST-SphI//NcoI-deGFP-T500 backbone to form pBEST-PX-UTRX-deGGFP-T500, where X = A, B, D, F, G, L, and LA.

Hypothesis 2: Gene L is necessary for phage propagation.

  • Need to design new primers (regular and mutagenic) for amplifying PHIX174 genome.


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SC 16:45, 25 June 2012 (EDT):