User:Sarah Burkhard/Notebook/471 Nano Notebook/2017/02/14

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Making pH 7 buffer

  • Made 50 ml sodium phosphate buffer solution for pH 7


exact mass of mono used: 0.1528 g

exact mass of di used: 0.2172 g

verified pH via pH meter: 7

Making amino acid solutions

We prepared 3 different amino acid stock solutions

1) Arginine

2) Cysteine

3) Glutamine

pH 7 [Au]=0.5 mM [AA]=3.125 uM Vf=3000 uL

Volumes for making the solution: XX uL AA XX uL Au XX uL HOH