User:Samiye Yaman/Notebook/Lab 571/2012/02/15

Biomaterials Design Lab

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Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers Objective -Synthesizing AuNP solutions. (purple solution) -UV-Vis of Solution 1 (Au/BSA mole ratio = 70) synthesized on 31/01/2012 [[1]] -Determining the effect of tris on solution 2 (Au/BSA mole ratio = 166) synthesized on 31/01/2012 [[2]] Description Synthesizing AuNP Combine 2 mL of 3.66 mM HAuCl4 and 6.04 mL of 0.0173 mM BSA in a test tube. Dilute to 20 mL with water. Place in oven at 80°C. Leave solutions in oven for 3 hr. Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on 01/31/12 over time. Determining the effect of tris buffer on 166 Au/BSA ratio solution Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12. Add 2 mL 50 mM tris buffer (pH 10.1) to solid protein fibers (no solution). Allow fibers to settle. Take UV-vis data of tris/fiber solution. Data