Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers
Objective
-Synthesizing AuNP solutions. (purple solution)
-UV-Vis of Solution 1 (Au/BSA mole ratio = 70) synthesized on 31/01/2012
[[1]]
-Determining the effect of tris on solution 2 (Au/BSA mole ratio = 166) synthesized on 31/01/2012
[[2]]
Description
Synthesizing AuNP
- Combine 2 mL of 3.66 mM HAuCl4 and 6.04 mL of 0.0173 mM BSA in a test tube.
- Dilute to 20 mL with water.
- Place in oven at 80°C.
- Leave solutions in oven for 3 hr.
- Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on 01/31/12 over time.
Determining the effect of tris buffer on 166 Au/BSA ratio solution
- Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12.
- Add 2 mL 50 mM tris buffer (pH 10.1) to solid protein fibers (no solution).
- Allow fibers to settle.
- Take UV-vis data of tris/fiber solution.
Data
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