User:Samiye Yaman/Notebook/Lab 571/2011/11/02
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3 different procedures were made.
1)Synthesizing gold nanoparticles.
-A test tube with 1 ml of BSA,7 ml of water and 2 ml of chloroauric acid.(with this order) was prepared -The test tube was placed in to the oven which was at 80 degree celcius and took it out of the oven for 15 min every 30 minutes.
- 10ul of DNA and 2ul of the loading buffer was placed in to a PCR tubes.
-10 ul of the sample was loaded to the gel and was run.
3) Preparing Agar Plate
- 0.875 g LB, 0.7g agar and 35ml of H2O were added to the 200 ml flask.
-The flask was autoclaved.
-All the agar solution was placed in to a petri dish with addition of 35ul of antibiotics.
-DNA was transferred into cells.
- The DNA was placed in to ice for 15min. - 35 ul of the cells and 5 ul of DNA were combined and incubated on ice for 30min. - Then it is placed heat shocked at 42C for 30 sec. - Incubated on ice for 5 min. - 250ul of SOC media was added. -incubated for an hour at 37C. -100uL of cells were spread on Lb/agar plate. -The plate was then inverted and stored in a 37C oven overnight.
Synthesizing Gold nanoparticles
t=0min : clear solution
t=30 min : formation of bubbles and formation of the yellow cloud
t=60min :the size of the yellow cloud shrink and it has a darker greenish yellow color. The bubbles are still present however there are less of them.
t=90min :formation of a greenish yellow solid. The bubbles disappeared.
t=120min : the greenish yellow solid shrank more and it is splinted in to two pieces.
t=150min: same as at 120min
LB agar plate -No growth of cells
This area is for any observations or conclusions that you would like to note.