User:SCGProject/Notebook/EBC 571/2021/10/26

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Coffee is considered the second most-consumed drink globally, and according to the literature, Brazil is the biggest coffee producer. There are many coffee species planted worldwide, and the main species are Coffea arabica and Robusta. Arabica has the best sensorial properties, such as flavor and aroma. On the other hand, Robusta is cheaper to produce. [14] The distinguished flavor and aroma of the coffee come from its chemical composition and consequently its price through trading and exporting. [16] Coffee customers become more acquainted with coffee’s quality and origin of what they drink. [8] According to market fundamentals, in July 2021, there were 10.7 million 60-Kg bags exported of all types of coffee by every exporting country to all importing countries. There was a slight increase of 1.7% in comparison to 10.5 bags in July 2020. However, in July 2019, before the COVID-19 pandemic hit, there were 11.9 million bags which was 4.4% above the latest production. However, coffee production is expected to grow after the easing of pandemic restrictions and consumption growth. [9] As a matter of fact, in This production and consumption of coffee, billions of tons of the spent coffee ground (SCG) goes to waste annually. Therefore, this enormous amount of biowaste can be helpful after recycling and reusing it in various ways such as soil fertilization, biorefinery, and neutralizing odors. Overall, SCG contains carbohydrates (45–47%, d.b.), proteins (13–17%, d.b.), lipids (9–16%, d.b.), and phenolic compounds (2–4%, d.b.) [7]

Many people worldwide consume coffee. If about 6 billion cups of coffee were consumed each year, around 93 percent of waste from coffee grounds would end up commonly disposed of via landfills [11]. The metabolic response of coffee trees in the rising atmospheric concentration of CO2 linked with the reduction in soil-water availability is complex due to the several (bio)chemical responses [19]. Organizations and research groups that suggest that SCG contributes to world contamination are currently looking for methods to value-add this waste product. Unfortunately, we still have a long way until we can efficiently face this current challenge. Pyrolysis methods have been implemented in SCG to extract high concentrations of fatty acids, alcohols, aldehydes, ketones, cellulose, hemicellulose, and lignin, concluding that coffee waste has excellent potential as a valuable bioenergy feedstock. [15]. Also, isolation compounds present in SCG are extracted with supercritical CO2 generally above 50 Celsius, 500 Atm, and different CO2 flow rates [2]. Additionally, magnetically modified spent coffee ground was successfully used to catalyze the aerobic oxidation of primary and secondary benzylic alcohols and tandem oxidative Groebke–Blackburn–Bienaymé reaction. [17] Finally, some applications on the whole SCG include from the preparation of PET/SCG composites [18] to analysis of its bioactive compounds as the whole composite with or without treatment [22]

Recent separation techniques have been implemented to isolate carbohydrates, lipids, and phenolic acids, while the protein fraction does not have a significant research interest at the moment. Carbohydrates have been extracted using easier processes, including autohydrolysis for polysaccharides [21]; constant shaking, heating, centrifugation, and filtration, followed by chloroform/methanol treatment [20]. The lipidic fraction was obtained with high yield percentages using absolute and hydrous ethanol. [5] On the other hand, Phenolic compounds in SCG can be used as antioxidants manufacturers such as food, cosmetics, and pharmaceuticals [23]. Its extraction has been determined using hydrothermal liquefaction [24], using the Folin-Ciocalteu reagent method readjusted to a 96-well microplate [21], as well as ultrasonic-assisted extractions (UAE) treatments with titanium alloy sonotrode [23]. Protein recovery from food byproducts improves the utilization of raw materials, reduces environmental pollution, and obtains beneficial proteins to human health. [25]. Research involving proteins in spent SGC includes methanol:H2O:acetic acid extraction [4], Kjeldahl method [7].

The arabica coffee light roasted bags from Ethiopia, Guatemala, Indonesia, Italy, and Papua New Guinea were purchased from different sellers in the eCommerce platform Amazon. [26] [27] [28] [29] [30]. The whole bean samples were grounded for thirty seconds using a home-quality grinder (KitchenAid BCG211). The extraction using 50g of coffee with 500mL of DI water was performed in a coffee maker (Cuisinart DCC-450BK) at 356.15°C and then oven-dried to constant mass 333.15 K for 16h (Ohaus PA84C Analytical Balance). The elemental analysis of each SCG was analyzed using a scanning electron microscope (JSM-IT100, SEM). Different UAEs were implemented to isolate each targeted group. LC-MS (Shimadzu LCMS-8045) analysis was essential to To complement the characterization of the specific molecules assigned to each group to verify that the new method to extract them was correctly achieved.


  • The following references are following ACS Style.
  1. Sulewska, A. M.; Larsen, F. H.; Sørensen, J. K.; Pedersen, A. H. Advanced Instrumental Characterization of the Coffee Extracts Produced by Pilot Scale Instant Coffee Process. Eur Food Res Technol 2021, 247 (6), 1379–1387. [1]
  2. Coelho, J. P.; Filipe, R. M.; Paula Robalo, M.; Boyadzhieva, S.; Cholakov, G. St.; Stateva, R. P. Supercritical CO2 Extraction of Spent Coffee Grounds. Influence of Co-Solvents and Characterization of the Extracts. The Journal of Supercritical Fluids 2020, 161, 104825. [2]
  3. Rijo, B.; Soares Dias, A. P.; Ramos, M.; de Jesus, N.; Puna, J. Catalyzed Pyrolysis of Coffee and Tea Wastes. Energy 2021, 235, 121252. [3]
  4. Valdés, A.; Castro-Puyana, M.; Marina, M. L. Isolation of Proteins from Spent Coffee Grounds. Polyphenol Removal and Peptide Identification in the Protein Hydrolysates by RP-HPLC-ESI-Q-TOF. Food Research International 2020, 137, 109368. [4]
  5. Toda, T. A.; Franco Visioli, P. de C.; de Oliveira, A. L.; da Costa Rodrigues, C. E. Conventional and Pressurized Ethanolic Extraction of Oil from Spent Coffee Grounds: Kinetics Study and Evaluation of Lipid and Defatted Solid Fractions. The Journal of Supercritical Fluids 2021, 177, 105332. [5]
  6. Williamson, K.; Hatzakis, E. NMR Analysis of Roasted Coffee Lipids and Development of a Spent Ground Coffee Application for the Production of Bioplastic Precursors. Food Research International 2019, 119, 683–692. [6]
  7. Rathnakumar, K.; Osorio-Arias, J. C.; Krishnan, P.; Martínez-Monteagudo, S. I. Fractionation of Spent Coffee Ground with Tertiary Amine Extraction. Separation and Purification Technology 2021, 274, 119111. [7]
  8. Markets, R. and. Global Coffee Market Report 2021-2026: Coffee Beans, Distribution Channel, Region, Market Insights, COVID-19 Impact, Competition and Forecast (accessed 2021 -09 -19). [8]
  9. International Coffee Organization - International Coffee Council (accessed 2021 -09 -10). [9]
  10. Chamyuang, S.; Owatworakit, A.; Intatha, U.; Duangphet, S. Coffee Pectin Production: An Alternative Way for Agricultural Waste Management in Coffee Farms. ScienceAsia 2021, 47S (1), 90. [10]
  11. These Two Entrepreneurs Are Shaking up Workplace Coffee Culture. ABC News. October 19, 2021. [11]
  12. Siqueira, J. H.; Santana, N. M. T.; Pereira, T. S. S.; Moreira, A. D.; Benseñor, I. M.; Barreto, S. M.; Velasquez-Melendez, G.; Molina, M. del C. B. Consumo de Bebidas Alcoólicas e Não Alcoólicas: Resultados Do ELSA-Brasil. Ciênc. saúde coletiva 2021, 26 (suppl 2), 3825–3837. [12]
  13. Motoki, K.; Takahashi, A.; Spence, C. Tasting Atmospherics: Taste Associations with Colour Parameters of Coffee Shop Interiors. Food Quality and Preference 2021, 94, 104315. [13]
  14. Cestari, A. Development of a Fast and Simple Method to Identify Pure Arabica Coffee and Blended Coffee by Infrared Spectroscopy. J Food Sci Technol 2021, 58 (9), 3645–3654. [14]
  15. Rijo, B.; Soares Dias, A. P.; Ramos, M.; de Jesus, N.; Puna, J. Catalyzed Pyrolysis of Coffee and Tea Wastes. Energy 2021, 235, 121252. [15]
  16. Rocha Baqueta, M.; Coqueiro, A.; Henrique Março, P.; Mandrone, M.; Poli, F.; Valderrama, P. Integrated 1H NMR Fingerprint with NIR Spectroscopy, Sensory Properties, and Quality Parameters in a Multi-Block Data Analysis Using ComDim to Evaluate Coffee Blends. Food Chemistry 2021, 355, 129618. [16]
  17. Farhid, H.; Shaabani, A. Magnetic Spent Coffee Ground as an Efficient and Green Catalyst for Aerobic Oxidation of Alcohols and Tandem Oxidative Groebke–Blackburn–Bienaymé Reaction. J IRAN CHEM SOC 2021, 18 (5), 1199–1209. [17]
  18. Mangindaan, D.; Lin, G.-Y.; Kuo, C.-J.; Chien, H.-W. Biosynthesis of Silver Nanoparticles as Catalyst by Spent Coffee Ground/Recycled Poly(Ethylene Terephthalate) Composites. Food and Bioproducts Processing 2020, 121, 193–201. [18]
  19. Marcheafave, G. G.; Tormena, C. D.; Mattos, L. E.; Liberatti, V. R.; Ferrari, A. B. S.; Rakocevic, M.; Bruns, R. E.; Scarminio, I. S.; Pauli, E. D. The Main Effects of Elevated CO2 and Soil-Water Deficiency on 1H NMR-Based Metabolic Fingerprints of Coffea Arabica Beans by Factorial and Mixture Design. Science of The Total Environment 2020, 749, 142350. [19]
  20. Desai, N. M.; Martha, G. S.; Harohally, N. V.; Murthy, P. S. Non-Digestible Oligosaccharides of Green Coffee Spent and Their Prebiotic Efficiency. LWT 2020, 118, 108784. [20]
  21. Ballesteros, L. F.; Teixeira, J. A.; Mussatto, S. I. Extraction of Polysaccharides by Autohydrolysis of Spent Coffee Grounds and Evaluation of Their Antioxidant Activity. Carbohydrate Polymers 2017, 157, 258–266. [21]
  22. Kovalcik, A.; Obruca, S.; Marova, I. Valorization of Spent Coffee Grounds: A Review. Food and Bioproducts Processing 2018, 110, 104–119. [22]
  23. Okur, I.; Soyler, B.; Sezer, P.; Oztop, M. H.; Alpas, H. Improving the Recovery of Phenolic Compounds from Spent Coffee Grounds (SCG) by Environmentally Friendly Extraction Techniques. Molecules 2021, 26 (3), 613. [23]
  24. Shiqi Zhang; Jie Yang; Sheng Wang; Vasantha Rupasinghe, H. P.; Quan He. Experimental Exploration of Processes for Deriving Multiple Products from Spent Coffee Grounds. Food and Bioproducts Processing 2021, 128, 21–29. [24]
  25. Du, Y.; Guo, X.; Han, Y.; Han, J.; Yan, J.; Shang, W.; Wu, H. Physicochemical and Functional Properties of Protein Isolate from Sea Cucumber ( Stichopus Japonicus ) Guts. J Food Process Preserv 2019, 43 (7). [25]
  26. : Copper Moon Guatemalan Antigua Blend, Whole Bean Coffee, 2 Pound Bag, Light Roast Coffee from Guatemala, Rich, Smooth, and Mild, with A Nutty Finish : Roasted Coffee Beans : Everything Else (accessed 2021 -09 -07).
  27. : The Organic Coffee Co. Sumatra Mandheling Whole Bean Coffee 2LB (32 Ounce) Medium Light Roast USDA Organic : Roasted Coffee Beans : Grocery & Gourmet Food (accessed 2021 -09 -07).
  28. : Caffe Mercanti Italian Roast Coffee Beans - Oro, the Perfect Boost to Feel More Energized (2.2 lb Bag) : Grocery & Gourmet Food (accessed 2021 -09 -07).
  29. : Raggiana Papua New Guinea Coffee Bean Light Roast, Organic Shade Grown Coffee with Rich Natural Flavor for Healthy and Fresh Morning Start (Ground for Drip, 12 Oz) : Grocery & Gourmet Food (accessed 2021 -09 -07).