User:Roberta Diaz Jimenez/Notebook/CHEM 471 Experimental Lab/2015/11/11

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Procedure

  • Ocean Optics
  1. Centrifuged falcon tubes containing gold fibers.
  2. Removed water with pipette.
  3. Prepare the sample that goes in the 3mL cuvette that will be used for ocean optics.
  4. The sample must contain:
    1. Gold fibers
    2. (2.996mL) of Tris buffer
    3. (0.003994mL) of trypsin, calculations are below
    4. The trypsin was added last, at the second reading such that second reading becomes time 0.
  5. We ran our sample overnight.
  6. Readings were taken every 20 minutes overnight. The data was uploaded directly to DropBox for later analysis.

Calculations for volume of trypsin, and volume of buffer

    C1·V1 = C2·V2,
     where (C1 = 50.21µM)
           C2 = 1µM 
           V2 = 1000µL
    (V1 = 0.0199mL) 
    C1·V1 = C2·V2,
     where (C1 = 1µM)
           C2 = 0.01µM 
           V2 = 3000µL
    (V1 = 30µL) 

Data

RAMOceanoptics 10nM.png

Graph OO 10 uM Trypsin.Abs vs time II.png

Graph OO 10 uM Trypsin.Abs vs time III.png

Results