User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/02/24

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Drosophila melanogaster Genetics Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Spermatogenesis

  • Dissected 7 sets of 5 gfp-myb, rfp-h2av males
    • Will use for anti-PCNA, anti-mip40, anti-mip120
    • Fixed 30' at RT
    • Wash 2 x 5' in 0.1% PBS-T
    • Incubate 25' in 0.6% PBS-T
    • Place in 1% NGS O/N