User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/11/16

Drosophila melanogaster Genetics	Main project page Previous entry      Next entry
Spermatogenesis Dissect tdt-Myb flies Fix 25' in 4% Formaldehyde in PBS Wash 3 x 5' in PBS-T Incubate 30' in PBS 0.6% Triton Rinse 1x with PBS-T Block O/N in PBS-T w/ 1% NGS. Tomorrow Stain with anti-alpha spectrin Incubate 30' with RNAse A and Toto