User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/11/16

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Spermatogenesis

  • Dissect tdt-Myb flies
    • Fix 25' in 4% Formaldehyde in PBS
    • Wash 3 x 5' in PBS-T
    • Incubate 30' in PBS 0.6% Triton
    • Rinse 1x with PBS-T
    • Block O/N in PBS-T w/ 1% NGS.
  • Tomorrow
    • Stain with anti-alpha spectrin
    • Incubate 30' with RNAse A and Toto