User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/09/28

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Spermatogenesis

  • Virgin control and experimental flies
    • Collect this week, set up Friday
  • Dissect Tdt-Myb
    • Dissected 13 testis.
    • Fixed 30 min in 4% formaldehyde PBS solution.
    • Washed 3x5' in PBST
    • Blocked 1 hr with 2% NGS solution.
    • Heather changed solution and incubated with 1:1000 rabbit anti-RFP at 4°C O/N.