User:Regina Vrikkis/Notebook/IL2 drug delivery/2012/05/16
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Attempt to make DSPC:DSPG (10:1) liposomes
DATE: 05/16/2012 Goal: Prepare DPPC liposomes to be incubated with rhIL-2. The following expt will be run.
[[ Materials and Methods:]] Materials Chloroform DPPC in chloroform 15ml tube Glass borisilicate tubes 100nm filters for extruder Filter supports (2) Extruder
Methods Thin film preparation 1. Work under hood when working with chloroform! 2. Rinse 10ml tube with chloroform. Do not need to dry b/c it will be evaporated later. 3. Add 1.1766ml DSPC (25mg/ml) in chloroform to tube. 4. Add 0.1223ml DSPG (25mg/ml) in chloroform to tube.
5. Evaporate chloroform to forma thin film by applying vacuum. 6. Dry film completely by vacuum desiccation ~1hr.