User:Raquel Lara/Notebook/project 1/2017/09/20

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Bradford Analysis BSA mass: 0.00543 BSA concentration= 0.108 mg/mL or 108 μg/ 1 mL Made 6 standard solutions between 1 μg/mL and 20 μg/mL (1) 108 μg/ 1 mL = 1μg/ x mL = 0.00925 mL or 9.25 μL (2) 108 μg/ 1 mL = 5μg/ x mL = 0.0462 mL or 46.2 μL (3) 108 μg/ 1 mL = 10μg/ x mL = 0.0925 mL or 92.5 μL (4) 108 μg/ 1 mL = 15μg/ x mL = 0.138 mL or 138 μL (5) 108 μg/ 1 mL = 20μg/ x mL = 0.185 mL or 185 μL (6) 108 μg/ 1 mL = 25μg/ x mL = 0.231 mL or 231 μL 200 μL of the Bio-Rad Protein Assay reagent added Tris/NaCl buffer added such that the final volume was 1 mL (1) 9.25 μL + 200 μL =209.25 μL , 1000 uL - 209.25 = 790.75 μL Tris/NaCl (2) 753.8 μL Tris/NaCl (3) 707.5 μL Tris/NaCl (4) 662 μL Tris/NaCl (5) 615 μL Tris/NaCl (6) 569 μL Tris/NaCl UV-VIS