User:Raquel Lara/Notebook/project 1/2017/08/30

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Preparation of Amino Acids with same concentration of BSA (250μM) 50 mL=0.05 L 0.05 L (250*10^-6 mol/1L) (165.2 g phenyl/1 mol) = 0.002 g phenyl 0.05 L (250*10^-6 mol/1L) (117.151 g valine/1 mol) = 0.0015 g valine 0.05 L (250*10^-6 mol/1L) (131.1729 g Isoleucine/1 mol) = 0.0016 g Isoleucine 0.05 L (250*10^-6 mol/1L) (174.2 g Argine/1 mol) = 0.00217 g Arginine Making AuNP using AA solution (1 mL/1000 mL) (250*10^-6 mol/1L) = 2.5*10^-6 mol Au 80:1 2.5*10^-6 mol Au (1mol AA/80 mol Au) = 3.125 *10^-8 mol AA 3.125 *10^-8 mol AA (1 L/ 250 *10^-6 mol AA) = 1.25 *10^-4 L = 125 μL AA 160:1 2.5*10^-6 mol Au (1mol AA/160 mol Au) = 1.56*10^-8 mol AA 1.56 *10^-8 mol AA (1 L/ 250 *10^-6 mol AA) = 6.25 *10^-4 L = 62.5 μL AA pH of Solutions -making sure it's close to the desired pH of 3.6 BSA 80:1 3.47 BSA 160:1 3.47 Valine 80:1 3.40 Valine 160:1 3.40 Phenyl 80:1 3.42 Phenyl 160:1 3.40 Isoleucine 80:1 3.41 Isoleucine 160:1 3.45