User:Puja Mody/Notebook/Magnetite Shenanigans/2014/09/24

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Magnetic Stimulus Testing

General Protocol:

  1. Centrifuge the 1.5 mL eppendorf tubes with the samples for 15 minutes at 13000 rpm
  2. Remove the R6G supernatant and store in clean eppendorf tub
  3. Add 1 mL deionized H2O to the eppendorf tube.
  4. Remove the deionized water. Repeat this rinsing process a minimum of three times
  5. Add 1.3 mL of Water to the eppendorf tube
  6. Place samples on UV/Fluorescence light to see if dye is present in hydrogel samples
  7. Centrifuge the samples in the same manner as in step 1.
  8. Remove this deionized water and place in a clean eppendorf tube for later testing ( in order to test if dye is coming out with centrifuging)
  9. Add 1.5 mL of water to sample
  10. Vortex the sample for ~2 minutes (until the sample is in homogeneous suspension)
  11. Apply magnet to the outside of the eppendorf tube for a minimum of 30 seconds. (until all the sample has aggregated
  12. Vortex sample again to re-suspend the hydrogels (at least 15 seconds)
  13. Re-apply magnet
  14. Complete this vortex/magnet process for 4 minutes
  15. Analyze the water using fluorescence

Analysis of the supernatant

  1. Place the supernatant in a clean cuvet and place into UV-Vis. Run UV-Vis analysis for 200-800nm.
  • If absorbance is too high, dilute samples in order to determine concentration of R6G remaining.