Magnetic Stimulus Testing
- Centrifuge the 1.5 mL eppendorf tubes with the samples for 15 minutes at 13000 rpm
- Remove the R6G supernatant and store in clean eppendorf tub
- Add 1 mL deionized H2O to the eppendorf tube.
- Remove the deionized water. Repeat this rinsing process a minimum of three times
- Add 1.3 mL of Water to the eppendorf tube
- Place samples on UV/Fluorescence light to see if dye is present in hydrogel samples
- Centrifuge the samples in the same manner as in step 1.
- Remove this deionized water and place in a clean eppendorf tube for later testing ( in order to test if dye is coming out with centrifuging)
- Add 1.5 mL of water to sample
- Vortex the sample for ~2 minutes (until the sample is in homogeneous suspension)
- Apply magnet to the outside of the eppendorf tube for a minimum of 30 seconds. (until all the sample has aggregated
- Vortex sample again to re-suspend the hydrogels (at least 15 seconds)
- Re-apply magnet
- Complete this vortex/magnet process for 4 minutes
- Analyze the water using fluorescence
Analysis of the supernatant
- Place the supernatant in a clean cuvet and place into UV-Vis. Run UV-Vis analysis for 200-800nm.
- If absorbance is too high, dilute samples in order to determine concentration of R6G remaining.