User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/02
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Horseradish Peroxidase Assay Absorbance
distilled water. It was then further diluted by taking 1mL of the solution in 50mL of 0.2 M sodium phosphate buffer.
Based on the other groups tests, we decided to keep all our variables constant except for the percent by volume of the AuNP added into each cuvet.The Gold Nanoparticle were made in a 1% citrate solution rather than in BSa. We decided that between the two separate trials, we would vary the concentration of the AAP because on the other groups results, it seems that the concentrations 156.25 mM and 312 mM were the most linear.
Because of changes in calculations and incorrect data results from the UV-Vis instrument, The spectra that we run today did not react in the manner in which we were looking for. However, please look at Mary's Notebook to see the spectras and analysis collected on this day.