User:Phillip R. Smith

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Phillip R. Smith

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Education

  • 2005-Present, PhD Candidate, Chemical Engineering, Stanford University
  • 2005, MS, Chemical Engineering, Brigham Young University
  • 2003, BS, Chemical Engineering, Brigham Young University

Papers

Bingham AS, Smith PR, Swartz JR. Evolution of an [FeFe] hydrogenase with decreased oxygen sensitivity. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.02.048

Smith PR, Bingham AS, Swartz JR. Generation of hydrogen from NADPH using an [FeFe] hydrogenase. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.03.172

Technical Presentations

<html><iframe src="http://player.vimeo.com/video/30534153?title=0&amp;byline=0&amp;portrait=0" width="400" height="225" frameborder="0" webkitAllowFullScreen mozallowfullscreen allowFullScreen></iframe><p><a href="http://vimeo.com/30534153">Phillip Smith | GCEP Symposium 2011</a> from <a href="http://vimeo.com/user3012557">Cyperus Media.com</a> on <a href="http://vimeo.com">Vimeo</a>.</p></html>

Research interests

  1. Protein Biotechnology (molecular engineering and directed protein evolution)
  2. Biochemical Reaction Engineering (bioprocess development)
  3. Synthetic Enzyme Pathways
  4. Protein Purification
  5. High-throughput Biotechnology

Biotechnology skillset

  • Protein overexpression (aerobic and anaerobic fermentation, shake-flask to 10L scale)
  • Protein purification (ion-exchange, affinity, and other chromatographic methods using an AKTA purifier and home-made systems)
  • Directed enzyme evolution
  • Protein engineering -- site directed mutagenesis, fusion protein design and construction
  • PCR, gene synthesis, mutagenesis
  • Spectrophotometric assay development

Useful links

Worth checking out