User:Paula Satisteban Vela

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Paula Santisteban Vela (an artistic interpretation)
  • Nury Paula Santisteban Vela
  • Memorial University of Newfoundland
  • St. John's, NL, Canada


  • 2019 Ph.D., Memorial University of Newfoundland, NL, Canada

Thesis title: Interaction of Antimicrobial Peptides with the Gram Positive and Gram Negative Cell Envelope
Supervisor: Valerie Booth. Co-Supervisor: Michael Morrow

    • During my time as a Ph.D. student, I developed and tested protocols for growing deuterium-rich bacteria to be able to perform deuterium NMR measurements in whole cells. I also tested the changes in the 2H NMR spectra of E. coli and B. subtilis in the presence of Antimicrobial peptides. Finally, I compared the 2H NMR spectra of B. subtilis with intact and compromised peptidoglycan layers.
  • 2013 MSc, Universidad De Los Andes, Bogotá

Thesis title: Mechanical properties of membranes as a factor of resistance to Antimicrobial Peptides
Supervisor: Chad Leidy

  • 2010, BSc, Universidad Nacional De Colombia

Research interests

  1. Biophysics
  2. Mechanics of Membrane
  3. NMR
  4. Antimicrobial Peptides
  5. NMR in whole cell


  1. Santisteban NP, Morrow MR, and Booth V. Effect of AMPs MSI-78 and BP100 on the lipid acyl chains of (2)H-labeled intact Gram positive bacteria. Biochim Biophys Acta Biomembr. 2020 May 1;1862(5):183199. DOI:10.1016/j.bbamem.2020.183199 | PubMed ID:31987866 | HubMed [Paper1]
  2. Booth V, Warschawski DE, Santisteban NP, Laadhari M, and Marcotte I. Recent progress on the application of (2)H solid-state NMR to probe the interaction of antimicrobial peptides with intact bacteria. Biochim Biophys Acta Proteins Proteom. 2017 Nov;1865(11 Pt B):1500-1511. DOI:10.1016/j.bbapap.2017.07.018 | PubMed ID:28844739 | HubMed [Paper2]
  3. Santisteban NP, Morrow MR, and Booth V. Protocols for Studying the Interaction of MSI-78 with the Membranes of Whole Gram-Positive and Gram-Negative Bacteria by NMR. Methods Mol Biol. 2017;1548:217-230. DOI:10.1007/978-1-4939-6737-7_15 | PubMed ID:28013507 | HubMed [Paper3]

    leave a comment about a paper here

All Medline abstracts: PubMed | HubMed

Events Participation

  1. Unraveling the Role of Peptidoglycan in the Interaction of Antimicrobial Peptides BP100 and MSI-78 with Bacterial Cell Envelopes through 2H NMR Experiments,Biophysical Journal, VOLUME 114, ISSUE 3, SUPPLEMENT 1, 454A, FEBRUARY 02, 2018. [1]
  2. 2H NMR Studies of Bacteria: How Does the Peptidoglycan Layer Modify the Interaction between Antimicrobial Peptides and the Lipid Membrane,Biophysical Journal, VOLUME 112, ISSUE 3, SUPPLEMENT 1, 380A-381A, FEBRUARY 03, 2017. [2]
  3. 2H NMR Studies of Living Bacteria Interacting with Antimicrobial Peptides,Biophysical Journal, VOLUME 110, ISSUE 3, SUPPLEMENT 1, 416A-417A, FEBRUARY 16, 2016. [3]
  4. The Antimicrobial Peptide Analog Magainin-H2 Induces Changes in the Phase Behavior and Lateral Organization of Supported Bilayers During Liquid-Crystalline/Gel Phase Coexistence,Biophysical Journal, VOLUME 102, ISSUE 3, SUPPLEMENT 1, 93A, JANUARY 31, 2012. [4]

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