User:Paul Rothenberg/Notebook/Pauls Notebook/2013/06/05

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Reaction of OPD+H2O2 with Mb/Citrate Catalyst in DMSO

Today, we are going back to running this reaction by dissolving the reactants, not suspending them. The same procedure will be followed as in previous reactions, just with DMSO as a solvent.

Data:


Mb Citrate OPD H2O2 DMSO 5C WORKUP GRAPH.png

The DMSO froze at 5°C. The graph indicated no absorbance at the final time point.

Mb Citrate OPD H2O2 DMSO 15C WORKUP GRAPH.png Mb Citrate OPD H2O2 DMSO 15C SEQUENTIAL WORKUP GRAPH.png

Mb Citrate OPD H2O2 DMSO 25C WORKUP GRAPH.png Mb Citrate OPD H2O2 DMSO 25C SEQUENTIAL WORKUP GRAPH.png

Mb Citrate OPD H2O2 DMSO 35C WORKUP GRAPH.png Mb Citrate OPD H2O2 DMSO 35C SEQUENTIAL WORKUP GRAPH.png

Mb Citrate OPD H2O2 DMSO 45C WORKUP GRAPH.png Mb Citrate OPD H2O2 DMSO 45C SEQUENTIAL WORKUP GRAPH.png

All of the reactions did not have as high of absorbances as Mb/Citrate in other solvents. There was also no defined peaks at 420 or 460 nm, as seen previously. Instead, the initial peak is at 400 nm, and that declines over the time interval. The curves are also not smooth, as compared to previous reactions. The same dip in the graph, seen just over 650 nm, was also seen yesterday when running the same reaction in suspension.

Reaction of OPD+H2O2 with Mb/Phosphate Catalyst in DMSO

Due to the DMSO freezing at 5°C, the reaction was only run at 25°C, 35°C, and 45°C.

Data:

Mb Phosphate OPD H2O2 DMSO 25C WORKUP GRAPH.png Mb Phosphate OPD H2O2 DMSO 25C SEQUENTIAL WORKUP GRAPH.png

Mb Phosphate OPD H2O2 DMSO 35C WORKUP GRAPH.png Mb Phosphate OPD H2O2 DMSO 35C SEQUENTIAL WORKUP GRAPH.png

Mb Phosphate OPD H2O2 DMSO 45C WORKUP GRAPH.png Mb Phosphate OPD H2O2 DMSO 45C SEQUENTIAL WORKUP GRAPH.png