User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/17
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100 nM Chymotrypsin In Situ Kinetics
10.4 μL of the stock was diluted with 1.99 mL Tris/NaCl/CaCl2 buffer.
Bradford Analysis of Thermolysin Reaction Samples
250 μL of each protein sample at every time point was combined with 200 μL of Bradford reagent (1:4 Bradford:Tris/CaCl2), and 550 mL of Tris/CaCl2 buffer and run from 400 - 800 nm on the UV/Vis spectrophotometer. The following are the results with the blank subtracted out and zeroed at a wavelength of 535 nm.