User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/17

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100 nM Chymotrypsin In Situ Kinetics

10.4 μL of the stock was diluted with 1.99 mL Tris/NaCl/CaCl2 buffer.

100nM Chymotrypsin AbsvsTime Mar17 Chart.png

100nM Chymotrypsin Kinetics Mar17 Chart.png

Bradford Analysis of Thermolysin Reaction Samples

250 μL of each protein sample at every time point was combined with 200 μL of Bradford reagent (1:4 Bradford:Tris/CaCl2), and 550 mL of Tris/CaCl2 buffer and run from 400 - 800 nm on the UV/Vis spectrophotometer. The following are the results with the blank subtracted out and zeroed at a wavelength of 535 nm.

Bradford kinetics 1uM thermolysin.png

Bradford kinetics 100nM thermolysin.png

Bradford kinetics 10nM thermolysin.png

Bradford kinetics 1nM thermolysin.png