User:Patrick Hampson/Notebook/chem471/2017/04/11

Project name

Main project page Previous entry      Next entry

Buffer solution preparation pH 10-13 pH 10: 21.4 mL of 0.1M NaOH + 100 mL of 0.05M NaHCO3 0.1 M NaOH created by adding 25 mL of 0.2M NaOH to 25 mL dI H2O 0.05 NaHCO3 made by addong 0.42005 grams of NaHCO3 to 100mL dI H2O pH 11: 8.2 mL of 0.1 NaOH + 100 mL of 0.05M Na2HPO4 0.1 M NaOH made by the same method as previously seen in the pH 10 buffer 0.05 M Na2HPO4 made by adding 0.7098 grams of Na2HPO4 to 100mL dI H2O pH 12: 12 mL of 0.2M NaOH + 50 mL of 0.2M KCL 0.2 M NaOH made by adding .8 grams of NaOH to 100mL of dI H2O 0.2 M KCl made by adding 1.491 grams of KCl to 100mL of dI H2O (this batch was split between pH 12 and 13 buffer solutions) pH 13: 132 mL of 0.2 NaOH + 50 mL of 0.2M KCl 0.2 M NaOH made by adding .8 grams of NaOH to 100mL of dI H2O 0.2 M KCl made by adding 1.491 grams of KCl to 100mL of dI H2O (this batch was split between pH 12 and 13 buffer solutions) Buffer solutions as actually measured by pH electrode: pH 10: 8.8 pH 11: 9.63 pH 12: 12.76 pH 13: 13.26 UV-Vis solution Preparation We then made solutions of pH 4-13 for Arginine (N-capped and uncapped), Glutamine, and Histidine Aspartic Acid solutions will be prepared tomorrow.