User:Patrick Hampson/Notebook/chem471/2017/02/21

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Today

  • Record results from pH 4, 6, and 7

Run UV-Vis on samples that were prepared last lab

Prepare samples for next lab

UV-Vis Results:

Arg ph4 absorbance.PNG Arg ph6 absorbance.PNG Arg ph7 absorbance.PNG

The graphs above contain the absorbance data from our Arginine pH 4, 6, and 7 solutions. As can be seen from the graphs above, Arginine had a rather lackluster formation of nanoparticles. pH 4 and 6 were relatively similar in the range of absorbance throughout. pH 6 had a relatively significant shoulder in the range of 300 nm. pH 7 followed a similar trend to pH 4, but had a slightly lower absorbance than either of the other two solutions.

Cys pH4 absorbance undiluted.PNG Cys pH6 absorbance.PNG Cys pH6 absorbance 0.5 dilution.PNG Cys pH6 absorbance 0.25 dilution.PNG Cys pH6 absorbance 0.125 dilution.PNG Cys pH7 absorbance.PNG

The graphs above contain absorbance data from our Cysteine pH 4, 6, and 7 solutions. All of the solutions had significant absorbance at 380 nm. This could be due to the precipitate that had formed in all of the solutions. Solution of pH 6 had significantly higher absorbance than the other two solutions at 380 nm, and required a dilution series. Upon dilution, a small shoulder appeared in the 350 nm, as well as in the 250 nm range.


Gln pH4 absorbance.PNG

Gln pH6 absorbance.PNG

Gln pH7 absorbance.PNG

The graphs above contain absorbance values for our Glutamine pH 4, 6, and 7 solutions. Soltuions of pH 4 and 7 did not create anything remarkable, and did not absorb significantly throughout the spectrum. The solution of pH 6 had higher absorbance than the other two solutions, and even had a small peak around 530 nm, which is were we expect a species of nanoparticles to appear.