User:Olivier Borkowski

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Contact Info

  • Olivier Borkowski, Paris, France

Current lab

  • Tenured Research Scientist (CRCN) INRAE, Université Paris-Saclay, AgroParisTech (Paris area, France)

Past lab experiences


  • 2013, PhD, Université de Paris
  • 2008, MS/Engineer degree, Graduate School of Life Sciences of Toulouse (ENSAT)

Research interests

  • Molecular Biology
  • Cell-free synthetic biology
  • Resource competition
  • Transcription/Translation processes
  • Feedback control system
  • Bacteria, Yeast
  • and more (or less)


  1. Borkowski O, Goelzer A, Schaffer M, Calabre M, Mäder U, Aymerich S, Jules M, and Fromion V. Translation elicits a growth rate-dependent, genome-wide, differential protein production in Bacillus subtilis. Mol Syst Biol. 2016 May 17;12(5):870. DOI:10.15252/msb.20156608 | PubMed ID:27193784 | HubMed [Paper1]
  2. Borkowski O, Gilbert C, and Ellis T. SYNTHETIC BIOLOGY. On the record with E. coli DNA. Science. 2016 Jul 29;353(6298):444-5. DOI:10.1126/science.aah4438 | PubMed ID:27471292 | HubMed [Paper2]
  3. Borkowski O, Ceroni F, Stan GB, and Ellis T. Overloaded and stressed: whole-cell considerations for bacterial synthetic biology. Curr Opin Microbiol. 2016 Oct;33:123-130. DOI:10.1016/j.mib.2016.07.009 | PubMed ID:27494248 | HubMed [Paper3]
  4. Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, Awan AR, Gilbert C, Stan GB, and Ellis T. Burden-driven feedback control of gene expression. Nat Methods. 2018 May;15(5):387-393. DOI:10.1038/nmeth.4635 | PubMed ID:29578536 | HubMed [Paper4]
  5. Borkowski O, Bricio C, Murgiano M, Rothschild-Mancinelli B, Stan GB, and Ellis T. Cell-free prediction of protein expression costs for growing cells. Nat Commun. 2018 Apr 13;9(1):1457. DOI:10.1038/s41467-018-03970-x | PubMed ID:29654285 | HubMed [Paper5]
  6. Koch M, Faulon JL, and Borkowski O. Models for Cell-Free Synthetic Biology: Make Prototyping Easier, Better, and Faster. Front Bioeng Biotechnol. 2018;6:182. DOI:10.3389/fbioe.2018.00182 | PubMed ID:30555825 | HubMed [Paper6]
  7. Koch M, Pandi A, Borkowski O, Batista AC, and Faulon JL. Custom-made transcriptional biosensors for metabolic engineering. Curr Opin Biotechnol. 2019 Oct;59:78-84. DOI:10.1016/j.copbio.2019.02.016 | PubMed ID:30921678 | HubMed [Paper7]
  8. Pandi A, Grigoras I, Borkowski O, and Faulon JL. Optimizing Cell-Free Biosensors to Monitor Enzymatic Production. ACS Synth Biol. 2019 Aug 16;8(8):1952-1957. DOI:10.1021/acssynbio.9b00160 | PubMed ID:31335131 | HubMed [Paper8]
  9. Borkowski O, Koch M, Zettor A, Pandi A, Batista AC, Soudier P, and Faulon JL. Large scale active-learning-guided exploration for in vitro protein production optimization. Nat Commun. 2020 Apr 20;11(1):1872. DOI:10.1038/s41467-020-15798-5 | PubMed ID:32312991 | HubMed [Paper9]
  10. Borkowski O, Drew E, Subsoontorn P. Hands-free control of heterologous gene expression in batch cultures (

  11. Synthetic Biology at the Hand of Cell-Free Systems (

  12. Advances and applications of cell-free systems for metabolic production (

  13. Nature Research Bioengineering Community: Active learning leads to highly efficient predictions in cell-free systems (

  14. EuSynBioS: Optimization of cell-free biosensors for synthetic biology (


All Medline abstracts: PubMed | HubMed

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