User:Norman Wang/Protocols

Cloning

• SLIC Cloning
• USER Cloning
• DNA Cloning Using In Vitro Site-Specific Recombination

DNA Preparation, Isolation & Purification

Preparation

• Alkaline Lysis Plasmid Mini-Prep

Isolation

• DNA Isolation Via Agarose Gel Filter+Membrane Extraction
• DNA Isolation Via Agarose Gel Two Well Extraction
• DNA Isolation Via Agarose Gel Band Cutting
• DNA Isolation Via Agarose Gel Electrolution

Purification

• Silica Membrane Spin Column DNA Purification
• Isopropanol DNA Precipitation

In Silico

• Primer Design for BioBricking
• BioBricking Primer Design Considerations