User:Nkuldell:M13 redesign

M13 redesign, expt'l details

Due Diligence

M13 phage proteins

phage proteins

phage particle cartoon

• NCBI link to seq: [[1]]
  
• Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[2]]
  

protein	aa (mature)	function	notes
I	348	assembly	includes gXI within, the two form structure in cytoplasmic membrane for phage maturation
II	410	replication of DNA + strand	includes gX within
III	406	phage tail protein (5 copies)	tag at N-term
IV	405	assembly	organizes to pore in OM
V	87	binds ssDNA	recognizes DNA PS
VI	112	phage tail protein (5 copies)	packages with pIII
VII	33	phage head protein (5 copies)	packages with p9
VIII	50	phage coat protein (2700 copies)	seg seq cleaved, matured form at aa 23 = clones to N-term, "Petrenko" site [[3]]
IX	32	phage head protein (5 copies)	stop codon overlaps start of gVIII
X	111	DNA replication	internal reinitiation inside gII
XI	108	assembly	translation reinitiation inside gI

Life Cycle of the Phage

cartoon depiction for life cycle of M13

key