User:Nkuldell/SAGA vs Swi/Snf
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in progress...
SAGA subunits, S. cerevisiae
most strains listed in Martens, Wu, Winston G&D paper 2005 [[1]]
link to FW yeast strain collection [[2]]
Parent strain for 109 deletion analysis: FY2068 = MAT(alpha) ura3-52 his3D200 leu2D1 lys2-128d
Ada subunits | size,chromosome,null p-type | deletion strain | genotype |
---|---|---|---|
Ada1 (aka HFI1, SUP110, SRM12, GAN1) [[3]] | 1.467 kb/489 aa, Chr. XVI, viable |
FY1560 aka NY383 |
MAT(A) ura3-52 his3D200 lys2-173R2 ada1::HIS3 |
Ada2 (aka SWI8) [[4]] | 1.305 kb/434aa, Chr. IV, viable |
FY1548 aka NY384 |
MAT(A) ura3-52 his3D200 leu2D1 lys2-128d ada2::HIS3 |
Ada3 (aka NGG1, SWI7) [[5]] | 2.109 kb/702aa, Chr. IV, viable |
FY1596 aka NY385 |
MAT(A) ura3-52 his3D200 his4-912d lys2-173R2 ada3::HIS3 |
Gcn5 (aka ADA4, SWI9) [[6]] | 1.32 kb/439aa, Chr. VII, viable |
FY1600 aka NY386 |
MAT(A) ura3-52 leu2D1 his3D200 his4-917d gcn5::HIS3 |
Ada5 (aka SPT20) [[7]] | 1.815 kb/604aa, Chr. XV, viable |
FY1098 | see spt20::URA3 |
Spt subunits | size, chromosome, null p-type | deletion strain | genotype |
---|---|---|---|
Spt3 [[8]] | 1.014 kb/337aa, Chr. IV, viable |
FY2142 aka NY387 |
MAT (alpha) spt3::KanMX |
Spt7 (aka GIT2) [[9]] | 3.999 kb/1332aa, Chr. II, viable |
FY963 aka NY388 |
MAT(A) ura3-52 his4-917d leu2d1 spt7::LEU2 |
Spt8 [[10]] | 1.809 kb/602aa, Chr. XII, viable |
FY50 aka NY389 |
MAT(A) ura3-52 his4-917d leu2d1 trp1d63 spt8d-302::LEU2 |
Spt20 (aka Ada5) [[11]] | 1.815 kb/604aa, Chr. XV, viable |
FY1098 aka NY390 |
MAT(A) ura3-52 his3d200 leu2d1 spt20d100::URA3 |
TAF subunits | size, chromosome, null p-type |
---|---|
TAF5 (aka TAF90) [[12]] | 2.397 kb/798aa, Chr. II, inviable |
TAF6 (aka TAF60) [[13]] | 1.551 kb/516aa, Chr. VII, inviable |
TAF9 (aka TAF17) [[14]] | 0.474 kb/157aa, Chr. XIII, inviable |
TAF10 (aka TAF23, TAF25) [[15]] | 0.621 kb/206aa, Chr. IV, inviable |
TAF12 (aka TAF61, TAF68) [[16]] | 1.620 kb/539aa, Chr. IV, inviable |
Tra1 subunit | size, chromosome, null p-type |
---|---|
Tra1 [[17]] | 11.235 kb/3744aa, Chr. VIII, inviable |
other subunits | size, chromosome, null p-type | deletion strain | genotype |
---|---|---|---|
Sgf73 [[18]] | 1.974 kb/657aa, Chr. VII , viable |
NY350 | MAT(A) ura3D0 his3D200 leu2D0 lys2-128d HAP1 DAN3:HIS3 sgf73::URA3 |
Sgf29 [[19]] | 0.779 kb/259aa, Chr. III, viable |
FY2474 | MAT(A) ura3-52 his4-917d leu2D1 lys2-173R2 arg4-12 sgf29D0::KanMX |
Sgf11 [[20]] | 0.3 kb/99aa, Chr.XVI, viable |
NY391 | MAT(A) ura3-52 his3D200 leu2D0 lys2-128d trp1-63 sgf11::KanMX |
Ubp8 [[21]] | 1.416 kb/471aa, Chr. XIII, viable |
FY2473 aka NY392 |
MAT(alpha) ura3-52 leu2D1 lys2-173R2 arg4-12 ubp8D0::KanMX |
Sus1 [[22]] | gene with intron, Chr. II, viable |
SWI/SNF subunits
SWI subunits | size,chromosome,null p-type |
---|---|
SWII (aka ADR6, GAM3, LPA1)[[23]] | 3945bp/1315 aa, Chr. XVI, inviable |
SWI2 (aka GAM1, HAF1, SNF2 , TYE3)[[24]] | 5112bp/1704aa, Chr. XV, viable |
SWI3 (aka TYE2) [[25]] | 2478bp/826aa, Chr. X, viable |
SNF subunits | size, chromosome, null p-type |
---|---|
SNF5 (aka HAF4, SWI10, TYE4)[[26]] | 2718 bp/906aa, Chr. II viable |
SNF6 [[27]] | 999bp/333aa, Chr. VIII, viable |
SNF11 [[28]] | 510 bp/170aa, Chr. IV viable |
SWP subunits | size, chromosome, null p-type |
---|---|
SWP82 | |
SWP73 | |
SWP59 | |
SWP61 | |
SWP29 |
Deletion Primers
- sgf73_URA3_KO_fwd (NO99)
- 5' TGAACACACAAGAGAAGCGCAAAAGAGTAAAGAGCTAAAatgtcgaaagctacatataa
- sgf73_URA3_KO_rev (NO100)
- 5' CTCACTTCGTGAACATGCTGGATAACGTGCATGATTCAA ttagttttgctggccgcatc
- sgf29_URA3_KO_fwd (NO101)
- 5' GACTTTTTCACAGCAAAACACACGGTCACCTTTCTTATTatgtcgaaagctacatataa
- sgf29_URA3_KO_rev (NO102)
- 5' AGAAGATCTTATGATATGTAGTAAATGTTAACCACCATTttagttttgctggccgcatc