User:Nishant M. Bhat/Protocols

Transforming chemically competent cells

Colony PCR (Endy)

Pouring LB plates (Endy)

Restriction Digests (Knight)

Streaking plates

3-way ligation (Bhat) (Adapted from the Endy protocol)

Making competent cells

Endy:Agarose_gel

Nmbhat:Antibiotic_cultures

Endy:PCR_Supermix

• dNTP dilution:
  • 108 H2O + 3 of each NTP (100mM)