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We prepare a 10 mM suspension of Taxol in DMSO. The Taxol is toxic, so it needs to be handled really carefully. This chemical comes in vials containing 170 µg. Therefore, to obtain a 10 mM solution we need to add 20 µl of DMSO. Once the solution is ready, vortex it and keep it in the electronic ice bucket or the fridge at all times.
Taxol in PEM
This is just the addition of 198.8 µL of PEM and 0.2 µL of the Taxol suspension in DMSO.