User:Nadiezda Fernandez-Oropeza/Notebook/Notebook/2010/11/16

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PEM-A

  • This entry contains the steps on preparing a suspension of ATP in PEM.

Preparation

The solution of ATP in PEM has to have a concentration of 100 mM. In this particular case, we prepare a total solution volume of 100 µl.

Then, the recipe is:

0.0051g of ATP

100 µl of PEM

Unfortunately, this is a very small amount of ATP and another way to proceed would be to place a small random amount of ATP in the wax paper, weigh it and figure out the amount of PEM necessary to obtain the 100 mM solution.

Finally, prepare 10 µl aliquots and place them in liquid Nitrogen as you finish aliquoting. Place them in the -80oC.