September 17, 2014
Calibration Curves for pH and Conductivity Continued
The remaining pH and conductivity measurements for serial dilutions 50x, 100x, 200x, 500x of the 50 mM NaCl solution, as well as for all 8 serial dilutions of the 50 mM glycine solution, were found:
Concentration of NaCl (mM)
|
pH
|
Conductivity (μS/cm)
|
50
|
8.827
|
5560
|
25
|
8.357
|
2924
|
10
|
8.199
|
1173
|
5
|
8.075
|
638
|
2.5
|
7.873
|
315
|
1.0
|
8.248
|
148.5
|
0.5
|
7.474
|
76.6
|
0.25
|
7.263
|
424
|
0.1
|
7.147
|
23.62
|
The following graphs demonstrate the collected data:
Concentration of Glycine (mM)
|
pH
|
Conductivity (μS/cm)
|
50
|
3.492
|
543
|
25
|
3.540
|
330
|
10
|
3.646
|
177.8
|
5
|
3.774
|
111.2
|
2.5
|
3.967
|
66.1
|
1.0
|
4.283
|
35.6
|
0.5
|
4.565
|
19.20
|
0.25
|
4.846
|
13.57
|
0.1
|
5.486
|
10.21
|
The following graphs demonstrate the collected data:
Baseline pH and conductivity measurements for 1g/L lysozyme stock solution pre-dialysis
Concentration of Lysozyme (g/L)
|
pH
|
Conductivity (μS/cm)
|
1
|
5.700
|
144.6
|
1:6 dilution
|
6.123
|
24.14
|
Electrophoresis Sample Preparation
5 samples were prepared and stored in the fridge for electrophoresis next week.
- 20 μL of the 1g/1L lysozyme stock solution were added to a 2 mL volumetric flask. 10 μL of this dilution were added to 10 μL of a running buffer prepared by Dr. Fox
- 20 μL of the 36.5:1 [Au]:[Lys] colloid solution were added to a 2 mL volumetric flask. 10 μL of this dilution were added to 10 μL of the running buffer
- 20 μL of a ___:__ [Au]:[BSA] colloid solution created the first day of lab were added to a 2 mL volumetric flask. 10 μL of this dilution were added to 10 μL of the running buffer
- 20 μL of a 1g/1L BSA stock solution were added to a 2 mL volumetric flask. 10 μL of this dilution were added to 10 μL of the running buffer
- 0.00209 g of powdered soy protein were dissolved in a 2 mL volumetric flask. 50 μL of this stock soy solution were then added to a 5 mL volumetric flask. 10 μL of this dilution were then added to 10 μL of the running buffer
Dialysis Sample Storage
The five dialysis samples prepared yesterday "soaked" overnight. Consequently, today they were removed from their respective soaks & tubing and placed in Falcon tubes for storage during the weekend, to be analyzed via Bradford assay next week.
NOTE: The lysozyme stock sample in 25K MWCO dialysis tubing (glycine soak) was spilled during transfer. The data for this sample will be obtained from another group.
|