User:Mischa L Spiegel/Notebook/Does DMSP have any effect on angiogenesis/

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Introduction

  • Observed and attempted removing tomato mouse endothelial cells (transformed with RFP)from cell culture
  • 30µl trypan blue added to 30µl cell culture and counted in haemocytometer = 2.3 x 10^5/ml cells
  • Portion of cells put back into cell culture
  • BCA assay conducted
  • Western gel made