User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/10/18
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ObjectiveTo make a labeled cysteine fluorophore and to measure the conductivity of the purple fibers previously created. Description
cysteine stock solution = 118 mg/mL cysteine molecular weight = 121.16 g/mol 5-(Iodoacetamido)fluorescein molecular weight = 515 g/mol
9.709 x 10-6 mol cysteine x 121.16 g / 1 mol= 1.18 x 10-3 g cysteine = 1.18 mg cysteine 1.18 mg cysteine / 3 = 0.39 mg cysteine (for 3x excess fluorescein) 0.39 mg x 1 mL / 118 mg stock solution = 3.3 uL stock added DataTest was not conducted because of broken probes. Notes |