User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/10/18
|Project name||Main project page|
Previous entry Next entry
To make a labeled cysteine fluorophore and to measure the conductivity of the purple fibers previously created.
cysteine stock solution = 118 mg/mL
cysteine molecular weight = 121.16 g/mol
5-(Iodoacetamido)fluorescein molecular weight = 515 g/mol
9.709 x 10-6 mol cysteine x 121.16 g / 1 mol= 1.18 x 10-3 g cysteine = 1.18 mg cysteine
1.18 mg cysteine / 3 = 0.39 mg cysteine (for 3x excess fluorescein)
0.39 mg x 1 mL / 118 mg stock solution = 3.3 uL stock added
Test was not conducted because of broken probes.