User:Michael F. Nagle/Notebook/Chem 571/2012/11/13

Project name

Main project page Previous entry      Next entry

Goals Analyze kinetics of Adenosine Deaminase with UV/Vis Analyze samples of various mole ratios of HRP to HAuCl4 prepared [by Dhea Patel] Procedure Dhea Patel measured the absorbance of HRP/Au samples with UV/Vis The procedure for an ADA Activity Assay was followed The buffer used was made with sodium phosphate rather than potassium phosphate. Puja Moody prepared the buffer at a pH of 7.4. The sodium and potassium phosphate buffers are interchangeable since they share the phosphate ion. Samples of Inosine, Adenosine and ADA in buffer were analyzed via UV/Vis Samples of Inosine, Adenosine and ADA in buffer were analyzed via UV/Vis Absorbance for adenosine maxed out the instrument, so the .1mM solution was further diluted with buffer to .5mM, which had absorbance near 5. Since this was still close to maxing out the machine, the adenosine was diluted to .05mM. Absorbance for 65μM ADA maxed out the instrument, so a serial dilution was completed 500μL ADA solution was transferred to 500μL sodium phosphate buffer, and 500μL of the resulting solution was transferred to 500μL. The transfer was repeated three times, producing solutions at the concentrations .195μM, 97.5μM, 48.75nM, and 24.38nM. Data