User:Michael F. Nagle/Notebook/Chem 571/2012/11/06

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Objectives Transform Adenosine Deaminase in cells Purify protein via Fast Protein Liquid Chromatography (FPLC) Procedure The prodedure for transformation was followed, just like on 10/24 but with the following modifications 20μL DNA solution was added to 30μL solution with E. Coli cells. 250μL Lysogeny Broth (LB) was used instead of SOC medium. Single 200μL plates were prepared. No 50μL plates were made. Plates were incubated at 37oC Adenosine Deaminase prepared Oct. 23 was purified via FPLC as was done 9/26 Pump flow was 5mL/minute