User:Meng Xiao He/Notebook/fall08/2008/12/12

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Replating on Gm, sucrose

  • colonies replated on both sucrose and Gm
  • 5 cbb and 6 cco colonies 20ul Phusion PCRed using PhFLANK program (12-8 modified to have 3:45 extension) > flanking primer pairs were only the ko gene and Duo as the control (no orientation primer)