User:Maureen McKeague

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Contact Info

Maureen McKeague

Maureen McKeague
Canada Research Chair in Functional Oligonucleotides, Tier II
Assistant Professor
Department of Pharmacology & Therapeutics, Faculty of Medicine and Health Sciences
Department of Chemistry, Faculty of Science
McGill University, Canada
Lab Website
Email me through OpenWetWare

Education

Research interests

I have been fascinated by nucleic acids since I learned about the basis of genetic inheritance. As a chemist, I view these biopolymers as functional and adaptable macromolecules with limitless potential. My collaborative nucleic acids research program integrates chemical biology, analytical chemistry, toxicology, and molecular biology. Major themes include: i) Developing clinical diagnostics tools and innovative biosensors; ii) Improving acute myeloid leukemia treatment; and iii) Enhancing the chemical diversity of nucleic acid interactions for therapeutic applications.

Publications

  1. Osman, E.A., Rynes, T.P.; Wang, Y.L.; Mruk, K.; McKeague, M. Non-invasive single cell aptasensing in live cells and animals. Chemical Science. 2024, 15, 4770-4778.
  2. Kovecses, O.; Mercier, F.E.; McKeague, M. Nucleic acid therapeutics as differentiation agents for myeloid leukemias. Leukemia. 2024, 38, 1441–1454.
  3. Rivera, M.; Ayon, O.S.; Diaconescu-Grabari, S.; Pottel, J.; Moitessier, N.; Mittermaier, A.; McKeague, M. A sensitive and scalable fluorescence anisotropy single stranded RNA targeting approach for monitoring riboswitch conformational states. Nucleic Acids Research. 2024, 52, 3164–3179.
  4. Ayon, O.S.; McKeague, M. Surface plasmon resonance assay for screening diverse aptamer-target interactions. Aptamers. 2023, 7, 7-12.
  5. DeRosa, M.C.; Lin, A.; Mallikaratchy, P.; McConnell, E.M.; McKeague, M.; Patel, R.; Shigdar, S. In vitro selection of aptamers and their applications. Nature Reviews Methods Primers. 2023, 3, 55.
  6. Maru, B.; Messikommer, A.; Huang, M.; Seipel, K.; Kovecses, O.; Valk, P.J.M.; Theocharides, A.P.A.; Mercier, F.E.; Pabst, T.; McKeague, M.; Luedtke, N. PARP-1 improves leukemia outcomes by inducing parthanatos during chemotherapy. Cell Reports Medicine. 2023, 4, 101191362.
  7. Jiang, Y.; Mingard, C.; Huber, S.; Takhaveev, V.; McKeague, M.; Kizaki, S.; Ziegler, N.; Schneider, M.; Hürlimann, V.; Hoeng, J.; Sierro, N.; Ivanov, N.; Sturla, S.J. Quantification and Mapping of Alkylation in the Human Genome Reveal Single Nucleotide Resolution Precursors of Mutational Signatures. ACS Central Science. 2023, 3, 362–372.
  8. McKeague, M.; Calzada, V.; Cerchia, L.; DeRosa, M.C.; Heemstra, J.M.; Janjic, N.; Johnson, P.E.; Kraus, L.; Limson, J.; Mayer, G.; Nilsen-Hamilton, M.; Porciani, D.; Sharma, T.K.; Suess, B.; Tanner, J.A.; Shigdar, S. The minimum aptamer publication standards (MAPS guidelines) for de novo aptamer selection. Aptamers. 2022.
  9. Forder, M.; McKeague, M. Tuning Aptamer-Switching for Biosensing Malarial Proteins. MSURJ. 2022.
  10. Robinson, K.A.; Lee, S.Y.; Friedman, S.; Christiaans, E.; McKeague, M.; Pavelka, L.; Sirjoosingh, P. You know what, I can do this”: Heterogeneous Joint Trajectories of Expectancy for Success and Attainment Value in Chemistry. Contemporary Educational Psychology. 2022. 69, 102055.
  11. Maru, B.; Nadeau, L.; McKeague, M. Enhancing CAR-T Cell Therapy with Functional Nucleic Acids. ACS Pharmacol. Transl. Sci. 2021,4, 6, 1716–17273.
  12. Bechu, A.; Liao, J.; Huang, C.; Ahn, C.; McKeague, M.; Ghoshal, S.; Moores, A. Cadmium-Containing Quantum Dots Used in Electronic Displays: Implications for Toxicity and Environmental Transformations. ACS Appl. Nano Mater. 2021. 8: 8417-8428.
  13. Hirka, S; McKeague, M. Quantification of aptamer-protein binding with fluorescence anisotropy Aptamers. 2021, 5.
  14. Krebs, J.; McKeague, M. Green toxicology: connecting green chemistry and modern toxicology Chemical Research in Toxicology. 2020, 33, 2919–2931.
  15. Mingard, C.; Wu, J.; McKeague, M.; Sturla, S.J. Next-generation DNA damage sequencing Chemical Society Reviews. 2020, 49, 7354-7377.
  16. Wang, L.; McKeague, M. Aptamers in the pursuit of COVID-19 management Aptamers. 2020, 4, 1-2.
  17. Oteng, E.K.; Gu, W.; McKeague, M. High-efficiency enrichment enables identification of aptamers to circulating Plasmodium falciparum-infected erythrocytes Scientific Reports. 2020, 10, 9706.
  18. Xiang, J.S.; Kaplan, M.; Dykstra, P.; Hinks, M.; McKeague, M.; Smolke, C.D. Massively Parallel RNA Device Engineering in Mammalian Cells with RNA-Seq and FACS-Seq Nature Communications. 2019. 10, 4327.
  19. Shigdar, S.; Johnson, P.E.; Pietruschka, G.; Legen, T.; Mayer, G.; McKeague, M. Aptamers 2019-a conference update Aptamers. 2019, 3, 1-3.
  20. Smith, M.; McKeague, M.; DeRosa, M.C. Synthesis, Transfer, and Characterization of Core-Shell Gold-Coated Magnetic Nanoparticles MethodsX. 2019.
  21. Kraus, A.; McKeague, M.; Seiwert, N.; Nagel, G.; Minneker, V.; Geisen, S. Zeigler, N.; Trantakis, I.A.; Kaina, B.; Thomas, A.D.; Sturla, S.J.; Fahrer, J. Immunological and mass spectrometry-based approaches to determine thresholds of the mutagenic DNA adduct O6-methylguanine in vivo, Arch Toxicol. 2019, 93, 559–572.
  22. Lam, J.; McKeague, M. Dietary modulation of mitochondrial DNA damage: Implications in ageing and associated diseases The Journal of Nutritional Biochemistry. 2019, 63, 1–10.
  23. McKeague, M.; Otto, C.; Angelov, T.; Räz, M.H.; Sturla, S.J. 2018. Base-pairing partner modulates alkylguanine alkyltransferase ACS Chemical Biology. 2018, 13 (9), 2534-2541.
  24. Wu, J.; McKeague, M.; Sturla, S.J. Nucleotide-resolution genome-wide mapping of oxidative DNA damage by click-code-seq, JACS. 2018, 140 (31), 9783-9787.
  25. DeGirolamo, A.;, McKeague, M.; Pascale, M.; Cortese, M.; DeRosa, M.C. Chapter 3: Immobilization of aptamers on substrates. Aptamers for Analytical Applications: Affinity Optimization and Method Design. Wiley-VCH Verlag GmbH & Co. (Ed) 2018, 85-126.
  26. Tanner, J.A.; Ismail, S.I.; Shigdar, S.; DeRosa, M.C.; Hahn, U.; Johnson, P.E.; Suess, B.; McKeague, M. Aptamers 2018-a conference update Aptamers. 2018, 2, 52-54.
  27. Dahlmann, H.A.; Berger, F.D.; Kung, R.W.; Wyss, L.A.; Gubler, I.; McKeague, M.; Wetmore, S.D.; Sturla, S.J. Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O6‐Alkylguanine Adducts Helvetica Chimica Acta.
  28. Dörsam, B.; Seiwert, N.; Foersch, S.; Stroh, S.; Nagel, G.; Begaliew, G.; Diehl, E.; Kraus, A.; McKeague, M.; Minneker, V.; Roukos, V.; Reißig, V.; Waisman, A.; Moehler, M.; Stier, A.; Mangerich, A.; Dantzer, F.; Kaina, B.; Fahrer, J. PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression, PNAS. 2018, 115, E4061-E4070.
  29. Awwad, A.M.; McKeague, M. Riboswitches and synthetic aptamers: a head-to-head comparison Aptamers. 2018, 2, 1-10.
  30. Henri, J.; McKeague, M.; Johnson, P.E.; Suess, B.; Nakamura, Y.; Nilsen-Hamilton, M.; Pastor, F.; Hahn, U.; Bunka, D.; Shigdar, S. Aptamers 2017 at Oxford Aptamers. 2018, 2, 11-14.
  31. Ruscito, A.; McConnell, E.M.; Koudrina, A.; Ranganathan, V.; Mattice, C.; Hunt, V.; McKeague, M.; DeRosa, M.C. In vitro selection and characterization of DNA aptamers to a small molecule target Current Protocols in Chemical Biology. 2017, 9, 233-268.
  32. McKeague, M. Aptamers for DNA damage and repair International Journal of Molecular Sciences. 2017, 18 (10), 2212.
  33. Alsaafin, A.; McKeague, M. Functional nucleic acids as in vivo metabolite and ion biosensors Biosensors and Bioelectronics. 2017, 94, 94–106.
  34. McKeague, M.; Ranganathan, V.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Smith, M.; DeRosa, M.C. Comparison of in-solution biorecognition properties of aptamers against ochratoxin A, Toxins. 2016, 8 (11), 336-348.
  35. Wang, Y-H.; McKeague, M.; Hsu, T.M.; Smolke, C.D. Design and construction of generalizable RNA-protein hybrid controllers by level-matched genetic signal amplification Cell Systems. 2016, 3 (6), 549–562.
  36. McKeague, M.; Wang, Y-H.; Cravens, A.; Win, M.N.; Smolke, C.D. Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines Metabolic Engineering. 2016, 38, 191–203.
  37. Valenzano, S.; De Girolamo, A.; DeRosa, M.C.; McKeague, M.; Schena, R.; Catucci, L.; Pascale, M. Screening and Identification of DNA aptamers to tyramine using in vitro selection and high-throughput sequencing. ACS Combinatorial Science. 2016, 18 (6), 302–313.
  38. McKeague, M.; Wong, R.S.; Smolke, C.D. Opportunities in the design and application of RNA for gene expression control Nucleic Acids Research. 2016, 44 (7),2987-2999
  39. McKeague, M.; Wang, Y-H.; Smolke, C.D. In vitro screening and in silico modeling of RNA-based gene expression control ACS Chemical Biology. 2015, 10 (11), 2463–2467.
  40. McKeague, M.; McConnell, E.M.; Cruz-Toledo, J.; Bernard, E.D.; Pach, A.; Zhang, X.; Beking, M.; Francis, T.; Giamberardino, A.; Cabecinha, A.; Ruscito, A.; Aranda-Rodriguez, R.; Dumontier, M.; DeRosa, M.C. Analysis of in vitro aptamer selection parameters. Journal of Molecular Evolution. 2015, 81(5), 150-161.
  41. Frost, N.R.; McKeague, M.; Falcioni, D.; DeRosa, M.C. An in solution assay for parallel interrogation of structure and affinity of small molecule-binding aptamers, Analyst. 2015, 140 (8) 6643–6651.
  42. McKeague, M.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Ruscito, A.; Ranganathan, V.; Frost, N.; Hill, K.; Smith, M.; McConnell, E.; DeRosa, M.C.Comprehensive analytical comparison of strategies used for small molecule aptamer evaluation, Analytical Chemistry. 2015, 87 (17), 8608–8612.
  43. Chang, A.L.; McKeague, M.; Smolke, C.D. Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.
  44. McKeague, M.; DeRosa, M.C. Aptamers and SELEX: Tools for the development of transformative molecular recognition technology, Aptamers and Synthetic Antibodies. 2014, 1 (1), 12-16.
  45. McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. Selection and characterization of a novel DNA aptamer For label-free fluorescence biosensing of ochratoxin A, Toxins. 2014, 6 (8), 2435-2452.
  46. Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. Kinetic and equilibrium binding characterization of aptamers to small molecules using a label-free, sensitive, and scalable Platform, Analytical Chemistry. 2014, 86 (7), 3273–3278.
  47. Mastronardi, E.; McKeague, M.; Monreal, C.; DeRosa, M.C. Development and application of crop exudate specific aptamers, Journal of Biomolecular Structure and Dynamics. 2013, 31 (1), 89.
  48. McKeague, M.; Foster, A.; Miguel, Y.; Giamberardino, A.; Verdin, C.; Chan, J.Y.; DeRosa, M.C. Development of a DNA aptamer for direct and selective homocysteine detection in human serum, RSC Advances. 2013, 3 (46), 24415-24422.
  49. McKeague, M.; DeRosa, M.C. Challenges and opportunities for small molecule aptamer development, Journal of Nucleic Acids. 2012.
  50. Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments. Database: Journal of Biological Databases and Curation. 2012.
  51. McKeague, M.; Giamberardino, A.; DeRosa, M.C. Advances in aptamer-based biosensors for food safety, Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech. DOI:10.5772/22350
  52. De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. Determination of ochratoxin A in wheat after clean-up through a DNA aptamer-based solid phase extraction column. Food Chemistry. 2011, 127 (3), 1378-1384.
  53. McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. Screening and initial binding assessment of fumonisin B(1) aptamers, International Journal of Molecular Sciences. 2010, 11 (12), 4864-4881.
  54. Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. Computational approaches toward the design of pools for the in vitro selection of complex aptamers, RNA. 2010, 16 (11), 2252-2262.

Leadership Roles/Memberships

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