User:Maureen McKeague

From OpenWetWare

Contact Info

Maureen McKeague

Maureen McKeague
Assistant Professor
Department of Pharmacology & Therapeutics, Faculty of Medicine
Department of Chemistry, Faculty of Science
McGill University
Canada
Email me through OpenWetWare

Education

Research interests

Genomic Chemistry: I have been fascinated by nucleic acids since I learned about the basis of genetic inheritance. As a chemist, I view these biopolymers as functional and adaptable macromolecules with limitless potential. As such, my research bridges the chemistry of the genome to natural product production and clinical diagnostics for new applications in green chemistry and cancer therapeutics.

Publications

  1. Lam, J.; McKeague, M. Dietary modulation of mitochondrial DNA damage: Implications in ageing and associated diseases The Journal of Nutritional Biochemistry. 2019, 63, 1–10.
  2. McKeague, M.; Otto, C.; Angelov, T.; Räz, M.H.; Sturla, S.J. 2018. Base-pairing partner modulates alkylguanine alkyltransferase ACS Chemical Biology. 2018, 13 (9), 2534-2541.
  3. Wu, J.; McKeague, M.; Sturla, S.J. Nucleotide-resolution genome-wide mapping of oxidative DNA damage by click-code-seq, JACS. 2018, 140 (31), 9783-9787.
  4. DeGirolamo, A.;, McKeague, M.; Pascale, M.; Cortese, M.; DeRosa, M.C. Chapter 3: Immobilization of aptamers on substrates. Aptamers for Analytical Applications: Affinity Optimization and Method Design. Wiley-VCH Verlag GmbH & Co. (Ed) 2018, 85-126.
  5. Tanner, J.A.; Ismail, S.I.; Shigdar, S.; DeRosa, M.C.; Hahn, U.; Johnson, P.E.; Suess, B.; McKeague, M. Aptamers 2018-a conference update Aptamers. 2018, 2.
  6. Dahlmann, H.A.; Berger, F.D.; Kung, R.W.; Wyss, L.A.; Gubler, I.; McKeague, M.; Wetmore, S.D.; Sturla, S.J. Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O6‐Alkylguanine Adducts Helvetica Chimica Acta.
  7. Dörsam, B.; Seiwert, N.; Foersch, S.; Stroh, S.; Nagel, G.; Begaliew, G.; Diehl, E.; Kraus, A.; McKeague, M.; Minneker, V.; Roukos, V.; Reißig, V.; Waisman, A.; Moehler, M.; Stier, A.; Mangerich, A.; Dantzer, F.; Kaina, B.; Fahrer, J. PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression, PNAS. 2018, 115, E4061-E4070.
  8. Awwad, A.M.; McKeague, M. Riboswitches and synthetic aptamers: a head-to-head comparison Aptamers. 2018, 2.
  9. Henri, J.; McKeague, M.; Johnson, P.E.; Suess, B.; Nakamura, Y.; Nilsen-Hamilton, M.; Pastor, F.; Hahn, U.; Bunka, D.; Shigdar, S. Aptamers 2017 at Oxford Aptamers. 2018, 2.
  10. Ruscito, A.; McConnell, E.M.; Koudrina, A.; Ranganathan, V.; Mattice, C.; Hunt, V.; McKeague, M.; DeRosa, M.C. In vitro selection and characterization of DNA aptamers to a small molecule target Current Protocols in Chemical Biology. 2017, 9, 233-268.
  11. McKeague, M. Aptamers for DNA damage and repair International Journal of Molecular Sciences. 2017, 18 (10), 2212.
  12. Alssafin, A.; McKeague, M. Functional nucleic acids as in vivo metabolite and ion biosensors Biosensors and Bioelectronics, 2017, 94, 94–106.
  13. McKeague, M.; Ranganathan, V.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Smith, M.; DeRosa, M.C. Comparison of in-solution biorecognition properties of aptamers against ochratoxin A, Toxins. 2016, 8 (11), 336-348.
  14. Wang, Y-H.; McKeague, M.; Hsu, T.M.; Smolke, C.D. Design and construction of generalizable RNA-protein hybrid controllers by level-matched genetic signal amplification Cell Systems. 2016, 3 (6), 549–562.
  15. McKeague, M.; Wang, Y-H.; Cravens, A.; Win, M.N.; Smolke, C.D. Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines Metabolic Engineering. 2016, 38, 191–203.
  16. Valenzano, S.; De Girolamo, A.; DeRosa, M.C.; McKeague, M.; Schena, R.; Catucci, L.; Pascale, M. Screening and Identification of DNA aptamers to tyramine using in vitro selection and high-throughput sequencing. ACS Combinatorial Science. 2016, 18 (6), 302–313.
  17. McKeague, M.; Wong, R.S.; Smolke, C.D. Opportunities in the design and application of RNA for gene expression control Nucleic Acids Research. 2016, 44 (7),2987-2999
  18. McKeague, M.; Wang, Y-H.; Smolke, C.D. In vitro screening and in silico modeling of RNA-based gene expression control ACS Chemical Biology. 2015, 10 (11), 2463–2467.
  19. McKeague, M.; McConnell, E.M.; Cruz-Toledo, J.; Bernard, E.D.; Pach, A.; Zhang, X.; Beking, M.; Francis, T.; Giamberardino, A.; Cabecinha, A.; Ruscito, A.; Aranda-Rodriguez, R.; Dumontier, M.; DeRosa, M.C. Analysis of in vitro aptamer selection parameters. Journal of Molecular Evolution. 2015, 81(5), 150-161.
  20. Frost, N.R.; McKeague, M.; Falcioni, D.; DeRosa, M.C. An in solution assay for parallel interrogation of structure and affinity of small molecule-binding aptamers, Analyst. 2015, 140 (8) 6643–6651.
  21. McKeague, M.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Ruscito, A.; Ranganathan, V.; Frost, N.; Hill, K.; Smith, M.; McConnell, E.; DeRosa, M.C.Comprehensive analytical comparison of strategies used for small molecule aptamer evaluation, Analytical Chemistry. 2015, 87 (17), 8608–8612.
  22. Chang, A.L.; McKeague, M.; Smolke, C.D. Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.
  23. McKeague, M.; DeRosa, M.C. Aptamers and SELEX: Tools for the development of transformative molecular recognition technology, Aptamers and Synthetic Antibodies. 2014, 1 (1), 12-16.
  24. McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. Selection and characterization of a novel DNA aptamer For label-free fluorescence biosensing of ochratoxin A, Toxins. 2014, 6 (8), 2435-2452.
  25. Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. Kinetic and equilibrium binding characterization of aptamers to small molecules using a label-free, sensitive, and scalable Platform, Analytical Chemistry. 2014, 86 (7), 3273–3278.
  26. Mastronardi, E.; McKeague, M.; Monreal, C.; DeRosa, M.C. Development and application of crop exudate specific aptamers, Journal of Biomolecular Structure and Dynamics. 2013, 31 (1), 89.
  27. McKeague, M.; Foster, A.; Miguel, Y.; Giamberardino, A.; Verdin, C.; Chan, J.Y.; DeRosa, M.C. Development of a DNA aptamer for direct and selective homocysteine detection in human serum, RSC Advances. 2013, 3 (46), 24415-24422.
  28. McKeague, M.; DeRosa, M.C. Challenges and opportunities for small molecule aptamer development, Journal of Nucleic Acids. 2012.
  29. Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments. Database: Journal of Biological Databases and Curation. 2012.
  30. McKeague, M.; Giamberardino, A.; DeRosa, M.C. Advances in aptamer-based biosensors for food safety, Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech. DOI:10.5772/22350
  31. De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. Determination of ochratoxin A in wheat after clean-up through a DNA aptamer-based solid phase extraction column. Food Chemistry. 2011, 127 (3), 1378-1384.
  32. McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. Screening and initial binding assessment of fumonisin B(1) aptamers, International Journal of Molecular Sciences. 2010, 11 (12), 4864-4881.
  33. Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. Computational approaches toward the design of pools for the in vitro selection of complex aptamers, RNA. 2010, 16 (11), 2252-2262.

Useful links