User:Matthew R Skorski/Notebook/471 - Exp BioChem/2016/04/05
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ObjectiveToday's objective is to
ProtocolMaking AuNP FibersBefore lab, I made 24 fiber samples, using the following reagents, in glass test tubes: I added the following to each sample:
I then put the samples in the oven and ran Program P1. In lab, we used these samples for our kinetics measurements. Making More AuNP Fiber Samples That FailedIn lab, we made an additional 32 AuNP fiber samples. I made new chloroauric acid for use in these fibers. However, I calculated the concentration of the chloroauric acid incorrectly, which I didn't realize until Wednesday (April 6). As a result, we did not add enough chloroauric acid to our samples (by a factor of about 100), and our samples did not form fibers (or even nanoparticles; they were all clear and colorless). We discarded the failed samples. Measuring the Kinetics of Rhodamine B In Equilibrium with AuNP FibersWe used the AuNP fiber samples that I synthesized before lab for these measurements. After the fibers came out of the oven, we added 71.94µM of the 1390µM stock of Rhodamine to each of 21 samples in order to bring the final concentration of Rhodamine in these samples 10 10µM. (The samples had mostly cooled at this point, but it's important to note that they were slightly warm in case this slight temperature change alters equilibrium conditions.) We covered all the samples with Parafilm, tilted them to mix the Rhodamine into solution, and then started a timer:
We saved all of the data into an Excel spreadsheet. Measuring the UV-Vis Absorption of AuNP Fiber Supernatants After Incubating in Rhodamine B for 2 HoursBefore lab, Michael pipetted the following amounts of Rhodamine into each of the samples that were synthesized on Wednesday of last week: After 2 hours, we spun down each sample for 2 minutes and pipetted 3.5mL of the supernatants into polystyrene cuvettes. We measured the UV-Vis absorption of each of these samples. ResultsKinetics of Rhodamine B In Equilibrium with AuNP FibersSee Michael's notebook UV-Vis Absorption of AuNP Fiber Supernatants After Incubating in Rhodamine B for 2 HoursSee Michael's notebook
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