User:Matthew R Skorski/Notebook/471 - Exp BioChem/2016/03/01
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Today's objectives are to:
We attempted to make a standard curve for the fluorescence of Rhodamine at a concentration of 0 to 1µM (with 0.1µM increments). However, our measurements did not make any sense. We suspect that we did not clean out the cuvettes well enough between each sample. Tomorrow, we will wash with more methanol between samples.
Start 540 nm End 700 nm Ex: 520 nm
Addition of Rhodamine to Samples Synthesized on February 24, 2016
We added Rhodamine to the samples that we synthesized on February 24, 2016 as follows:
Note that the samples ending in _04 were blanks (we did not add any Rhodamine to these samples).
AuNP Fiber Synthesis
We made new AuNP fiber samples as follows:
We incubated the samples in the oven at 80 degrees Celsius for four hours.
Data, Analysis, and Observations
We will take fluorescence measurements of all of our samples tomorrow. See the entry for March 2, 2016 for the data and analysis.