User:Matt Hartings/Notebook/Photosynthesis/2013/05/06

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Mb in MeOH Prepping samples for visit to NIST tomorrow to run Circular Dichroism Methanol From citrate 0.1011g of (5.7mg Mb lyophilized from 20mL of 10mM citrate buffer, pH 3, with 0.4861g KCl) 10mL methanol Vortex for 2 minutes centrifuge at 4500rpm for 15 minutes From acetate 0.1010g of (3.0mg Mb lyophilized from 20mL of 10mM acetate buffer, pH 5, with 0.4856g KCl) 10 mL methanol Vortex for 2 minutes centrifuge at 4500rpm for 15 minutes From phosphate 0.1079g of (5.8mg Mb lyophilized from 20mL of 10mM phosphate buffer, pH 7, with 0.4961g KCl) 10 mL methanol Vortex for 2 minutes centrifuge at 4500rpm for 15 minutes Water From citrate 0.0538g of (5.7mg Mb lyophilized from 20mL of 10mM citrate buffer, pH 3, with 0.4861g KCl) 5.3mL water From acetate 0.0525g of (3.0mg Mb lyophilized from 20mL of 10mM acetate buffer, pH 5, with 0.4856g KCl) 5 mL water From phosphate 0.0531g of (5.8mg Mb lyophilized from 20mL of 10mM phosphate buffer, pH 7, with 0.4961g KCl) 5 mL water