User:Matt Hartings/Notebook/Photosynthesis/2013/03/19

Protein Re-engineering

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Ocean Optics Procedure Figuring out procedure for collecting kinetics data with ocean optics software. Save Reference spectrum file Light source on Blank Cuvette with buffer in cuvette holder Select File|Store|Store Reference Spectrum Save Dark spectrum file Block light path Select File|Store|Store Dark Spectrum Saving multiple spectra over a time course Select File|New|High Speed Aquisition Integration Time (μs): 10,000 (10ms) Scans to Average: 1 Boxcar width: 0 External Trigger Mode: Normal Filename ---> Select Number of scans 5000 (this could be huge. might need to rethink ...) Prepping Mixer Nyousha made 200mL of 50mM tris 10mM sodium dithionite, pH 8 and is filtering it. We are going to bubble nitrogen through it and then purge the mixer with this solution to degas the mixer and get rid of the oxygen.