User:Matt Hartings/Notebook/Photosynthesis/2013/02/26

From OpenWetWare
Jump to navigationJump to search
Protein Re-engineering Main project page
Previous entry      Next entry

Protein Dialysis

In order to prepare the Mn-Hb samples for purification, I am dialyzing the samples.

  1. Make 4L of 10mM Tris 50mM NaCl, pH 8.5
    1. Measured 11.681 g NaCl
    2. Measured 4.860 g Tris
  2. I split the crude Mn-Hb solution over 2 dialysis tubes (snakeskin).
  3. The dialysis tubes were places in the buffer and am storing them in the cold room overnight