User:Matt Hartings/Notebook/Photosynthesis/2013/01/18

Protein Re-engineering

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Objective Measure stock DNA solutions Procedure Dilute samples (2 uL of DNA, 198 uL of water) Measure absorbance. Assume: for dsDNA, an absorbance of 1 corresponds to a concentration of 50ug/mL and for oligos an absorbance of 1 corresponds to a concentration of 20ug/mL When I could, I used data sheets from IDT for epsilon values of oligos Data Important notes: I need to do new mini/midi-preps for WT sw Mb and pQE-80 as the concentrations were just so low. Asc Hb T109C f 136ug/mL (from assumption) Asc Hb T109C r 272ug/mL (from assumption) sw Mb M131T f 60.5 mmol/mL (from data sheet) sw Mb M131T r 10.3 mmol/mL (from data sheet) sw Mb pQE80 clone f 95.1 mmol/mL (from data sheet) sw Mb pQE80 clone r 30.4 mmol/mL (from data sheet)