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Matt Hartings/Notebook/Photosynthesis/2012/09/13
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Notebook
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Photosynthesis
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2012
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09
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Protein Re-engineering
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Objective
Extract Hb protein from cells.
Procedure
Thaw cell pellets
Run 3 cycles of ultrasonication
30seconds sonicating
30 seconds on ice
Balance centrifuge tubes (oak ridge tubes) containing sonicated cells with water
centrifuge at 18000rpm at 4C for 2 hours
decant liquid from tubes into fresh falcon tubes.
store in refrigerator
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