User:Matt Hartings/Notebook/Photosynthesis/2012/09/13

From OpenWetWare
Jump to navigationJump to search
Hartings AU Photosynthesis Lab Header.png Protein Re-engineering Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png



Objective

Extract Hb protein from cells.

Procedure

  1. Thaw cell pellets
  2. Run 3 cycles of ultrasonication
    1. 30seconds sonicating
    2. 30 seconds on ice
  3. Balance centrifuge tubes (oak ridge tubes) containing sonicated cells with water
  4. centrifuge at 18000rpm at 4C for 2 hours
  5. decant liquid from tubes into fresh falcon tubes.
  6. store in refrigerator