User:Matt Hartings/Notebook/Photosynthesis/2012/09/11

Biomaterials Design Lab

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Objective Prep for hemoglobin expression tomorrow Procedure Prepare autoclaved LB broth Place 25 g of LB in Fernbach flask Add 1 L of water Repeat to make a total of 4 flasks Place 0.875g of LB in 250mL ehrlenmeyer flask Add 35mL of water Repeat to make a total of 4 flasks Autoclave flasks 2 runs each containing 2 fernbachs and 2ehrlenmeyers Inoculate starter cultures Add 35uL of 100mg/mL ampicilin to each ehrlenmeyer Transfer culture from frozen glycerol stock of WT Ascaris Hb to starter culture flasks Shake overnight at 37C