User:Matt Hartings/Notebook/Photosynthesis/2012/06/25

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Objective

Extract hemoglobin from Ecoli previously used in expression.

Description

  1. Thaw resuspended cells
  2. Sonicate at power level 12 on Misonix XL-2000. Sonicate for 30 seconds. Rest on ice for 30 seconds.
  3. Place in centrifuge tubes and balance weight to near 0.01g
  4. Centrifuge for 2 hours at 18000rpm and 4C
  5. Dialyze into 25mM Tris, 50mM NaCl pH 8.3 overnight in cold room.

Data

  • Add data and results here...

Notes

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