User:Matt Hartings/Notebook/Photosynthesis/2012/06/25

Biomaterials Design Lab

Main project page Previous entry      Next entry

Objective Extract hemoglobin from Ecoli previously used in expression. Description Thaw resuspended cells Sonicate at power level 12 on Misonix XL-2000. Sonicate for 30 seconds. Rest on ice for 30 seconds. Place in centrifuge tubes and balance weight to near 0.01g Centrifuge for 2 hours at 18000rpm and 4C Dialyze into 25mM Tris, 50mM NaCl pH 8.3 overnight in cold room. Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.