User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/29

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Objective

Calculations for stock solutions to be made tomorrow.

Description

stock solutions

  1. 1 L of 0.001 M HCl
    1. Concentrated HCl should be: 11.65 M
    2. M1V1 = M2V2
    3. (11.65 M)(V1) = (0.001M)(1L)
    4. V1 = 85.8 μL
  2. 1 L of 0.001 M HNO3
    1. Concentrated HNO3 should be: 15.8 M
    2. M1V1 = M2V2
    3. (15.8 M)(V1) = (0.001M)(1L)
    4. V1 = 63.2 μL
  3. 1 L of 1 M NaOH
    1. MW of NaOH = 39.997 g/mol
    2. M = (m/MW)/V
    3. 1 M = (m/39.997 g/mol)/1L
    4. m = 39.997 g
  4. 1 L of 0.001 M NaOH
    1. Use 1M stock solution
    2. M1V1 = M2V2
    3. (1 M)(V1) = (0.001M)(1L)
    4. V1 = 1 mL
  5. 10 mL of 12.5 mM Fructose
    1. MW = 180.16 g/mol
    2. 0.0125 M = (m/180.16 g/mol)/(0.010 L)
    3. m = 22.5 mg
  6. 10 mL of 2.5 mM AuCl3
    1. MW = 303.33 g/mol
    2. 0.0025 M = (m/303.33 g/mol)/(0.010 L)
    3. m = 7.58 mg
  7. Protein (BSA)
    1. MW = 66776 g/mol
    2. 30 μM = (m/66776 g/mol)/(0.010 L)
    3. m = 20.0 mg

sample solutions

  1. 0.25 mM AuCl3, 0 mM fructose, pH 4 ([H+] = 1x10-4 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. Vfructose stock = 0 mL
    3. Volume of HCl stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-4 M)(5 mL)
      3. VHCl stock = 0.5 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
      2. Vwater = 3.483 mL
  2. 0.25 mM AuCl3, 0 mM fructose, pH 5 ([H+] = 1x10-5 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. Vfructose stock = 0 mL
    3. Volume of HCl stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-5 M)(5 mL)
      3. VHCl stock = 0.05 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
      2. Vwater = 3.933 mL
  3. 0.25 mM AuCl3, 0 mM fructose, pH 6 ([H+] = 1x10-6 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. Vfructose stock = 0 mL
    3. Volume of HCl stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-6 M)(5 mL)
      3. VHCl stock = 0.005 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
      2. Vwater = 3.978 mL
  4. 0.25 mM AuCl3, 0 mM fructose, pH 7 ([H+] = 1x10-7 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. Vfructose stock = 0 mL
    3. Volume of HCl stock
      1. VHCl stock = 0 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
      2. Vwater = 3.983 mL
  5. 0.25 mM AuCl3, 0 mM fructose, pH 8 ([H+] = 1x10-8 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. Vfructose stock = 0 mL
    3. Volume of 0.001 M NaOH stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-6 M)(5 mL)
      3. VNaOH stock = 0.005 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
      2. Vwater = 3.978 mL
  6. 0.25 mM AuCl3, 0 mM fructose, pH 9 ([H+] = 1x10-9 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. Vfructose stock = 0 mL
    3. Volume of 0.001 M NaOH stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-5 M)(5 mL)
      3. VNaOH stock = 0.05 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
      2. Vwater = 3.933 mL
  7. 0.25 mM AuCl3, 0 mM fructose, pH 10 ([H+] = 1x10-10 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. Vfructose stock = 0 mL
    3. Volume of 0.001 M NaOH stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-4 M)(5 mL)
      3. VNaOH stock = 0.5 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
      2. Vwater = 3.483 mL
  8. 0.25 mM AuCl3, 0 mM fructose, pH 11 ([H+] = 1x10-11 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. Vfructose stock = 0 mL
    3. Volume of 1 M NaOH stock
      1. M1V1 = M2V2
      2. (1 M)V1 = (1x10-3 M)(5 mL)
      3. VNaOH stock = 0.005 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
      2. Vwater = 3.978 mL
  9. 0.25 mM AuCl3, 0 mM fructose, pH 12 ([H+] = 1x10-12 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. Vfructose stock = 0 mL
    3. Volume of 1 M NaOH stock
      1. M1V1 = M2V2
      2. (1 M)V1 = (1x10-2 M)(5 mL)
      3. VNaOH stock = 0.05 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
      2. Vwater = 3.933 mL
  10. 0.25 mM AuCl3, 0 mM fructose, pH 4 ([H+] = 1x10-4 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. M1V1 = M2V2
      2. (0.0125 M)V1 = (0.0000625 M)(5 mL)
      3. Vfructose stock = 0.025 mL
    3. Volume of HCl stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-4 M)(5 mL)
      3. VHCl stock = 0.5 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
      2. Vwater = 3.458 mL
  11. 0.25 mM AuCl3, 0 mM fructose, pH 5 ([H+] = 1x10-5 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. M1V1 = M2V2
      2. (0.0125 M)V1 = (0.0000625 M)(5 mL)
      3. Vfructose stock = 0.025 mL
    3. Volume of HCl stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-5 M)(5 mL)
      3. VHCl stock = 0.05 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
      2. Vwater = 3.908 mL
  12. 0.25 mM AuCl3, 0 mM fructose, pH 6 ([H+] = 1x10-6 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. M1V1 = M2V2
      2. (0.0125 M)V1 = (0.0000625 M)(5 mL)
      3. Vfructose stock = 0.025 mL
    3. Volume of HCl stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-6 M)(5 mL)
      3. VHCl stock = 0.005 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
      2. Vwater = 3.953 mL
  13. 0.25 mM AuCl3, 0 mM fructose, pH 7 ([H+] = 1x10-7 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. M1V1 = M2V2
      2. (0.0125 M)V1 = (0.0000625 M)(5 mL)
      3. Vfructose stock = 0.025 mL
    3. Volume of HCl stock
      1. VHCl stock = 0 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VHCl stock - VProtein Stock
      2. Vwater = 3.958 mL
  14. 0.25 mM AuCl3, 0 mM fructose, pH 8 ([H+] = 1x10-8 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. M1V1 = M2V2
      2. (0.0125 M)V1 = (0.0000625 M)(5 mL)
      3. Vfructose stock = 0.025 mL
    3. Volume of 0.001 M NaOH stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-6 M)(5 mL)
      3. VNaOH stock = 0.005 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
      2. Vwater = 3.953 mL
  15. 0.25 mM AuCl3, 0 mM fructose, pH 9 ([H+] = 1x10-9 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. M1V1 = M2V2
      2. (0.0125 M)V1 = (0.0000625 M)(5 mL)
      3. Vfructose stock = 0.025 mL
    3. Volume of 0.001 M NaOH stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-5 M)(5 mL)
      3. VNaOH stock = 0.05 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
      2. Vwater = 3.908 mL
  16. 0.25 mM AuCl3, 0 mM fructose, pH 10 ([H+] = 1x10-10 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. M1V1 = M2V2
      2. (0.0125 M)V1 = (0.0000625 M)(5 mL)
      3. Vfructose stock = 0.025 mL
    3. Volume of 0.001 M NaOH stock
      1. M1V1 = M2V2
      2. (1x10-3 M)V1 = (1x10-4 M)(5 mL)
      3. VNaOH stock = 0.5 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
      2. Vwater = 3.458 mL
  17. 0.25 mM AuCl3, 0 mM fructose, pH 11 ([H+] = 1x10-11 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. M1V1 = M2V2
      2. (0.0125 M)V1 = (0.0000625 M)(5 mL)
      3. Vfructose stock = 0.025 mL
    3. Volume of 1 M NaOH stock
      1. M1V1 = M2V2
      2. (1 M)V1 = (1x10-3 M)(5 mL)
      3. VNaOH stock = 0.005 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
      2. Vwater = 3.953 mL
  18. 0.25 mM AuCl3, 0 mM fructose, pH 12 ([H+] = 1x10-12 M), 5 mL total
    1. Volume of AuCl3 stock
      1. M1V1 = M2V2
      2. (0.0025 M)V1 = (0.00025 M)(5 mL)
      3. VAuCl3 stock = 0.5 mL
    2. Volume of fructose stock
      1. M1V1 = M2V2
      2. (0.0125 M)V1 = (0.0000625 M)(5 mL)
      3. Vfructose stock = 0.025 mL
    3. Volume of 1 M NaOH stock
      1. M1V1 = M2V2
      2. (1 M)V1 = (1x10-2 M)(5 mL)
      3. VNaOH stock = 0.05 mL
    4. Volume of Protein stock
      1. M1V1 = M2V2
      2. (30 μM)VProtein Stock = (3.1 μM))(5 mL)
      3. VProtein Stock = 0.517 mL
    5. Volume of water
      1. Vwater = 5 mL - VAuCl3 stock - Vfructose stock - VNaOH stock - VProtein Stock
      2. Vwater = 3.908 mL

Data

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Notes

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