User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/12

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Transform BL21(DE3) cells with the DNA extracted yesterday.


Following transformation protocol I normally use:

  1. Place plastic culture tubes on ice for 15 minutes
  2. Add cells to ice to thaw
  3. Add 1uL of DNA to tubes
  4. Place on ice for 1 minute
  5. Place 30uL of cells over DNA
  6. Incubate for 4 minutes
  7. Heat shock for 80 seconds
  8. Put cells on ice
  9. Add 100uL of SOC
  10. Shake at 37C for 1 hr
  11. Plate on kanamycin LB plates


  • Add data and results here...


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