User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2011/12/13
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Checking sequences for the GFP mutants today. The PCR was previously run on the wild-type to mutate the aspartate at position 1 to a cysteine. We tried this in class previously, but the primers that I ordered had no mutations in them. New primers were ordered and PCR was run with the following conditions: two reaction vials were started
Each reaction was allowed to proceed for 10 cycles. After 10 cycles the two reaction containers were mixed.
The sequence result can be found here
This area is for any observations or conclusions that you would like to note.