User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2011/10/04

Biomaterials Design Lab

Main project page Previous entry      Next entry

Objective Today and tomorrow we will be determining the fluorescence of MBP as a function of concentration. To do this, you will need to be able to know the concentration of MBP based on its absorbance spectrum. So, if you still don't have that figured out properly from our Bradford reagent lab, you will need to get this figured out during the lab time this week. Also, you will need to figure out the sensitivity of our new fluorescence instrument. That is, at what intensity does the fluorescence intensity not vary linearly with concentration. To determine this, you will need to make standard solutions of Ru(bpy)(phen) in acetonitrile. Note: To get an accurate determination of fluorescence intensity, you don't measure just the intensity at the emission peak. You measure the integrated intensity of the entire emission. This will be an excel-heavy lab. Description Add experimental record here. Include what, how, and why... Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.