User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2011/08/31

Biomaterials Design Lab

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Objective Making buffers for class Description Add experimental record here. Include what, how, and why... Data Buffers Stock solutions: 1.8M Acetic Acid Media: Sodium Acetate Tris-Aquo Media: Tris Base Moles of each calculated using the buffer calculator for buffers made and used at 25C Buffer 1 Water Buffer 2 100 mL of 50mM Acetate Buffer pH 3.76 Calculations Amount of acid component M=nV (1.8M)=(0.0045mol)(V) V=2.5mL Amount base component m=MM*n m=136.08g/mol*0.00048mol m=0.0544g Actual measurement = 0.0563g Actual pH=3.6 Buffer 3 100 mL of 50mM Acetate Buffer pH 4.5 Calculations Amount of acid component M=nV (1.8M)=(0.00307mol)(V) V=1.67mL Amount base component m=MM*n m=136.08g/mol*0.00192mol m=0.259g Actual measurement = 0.276g Actual pH=4.3 Buffer 4 100 mL of 50mM Acetate Buffer pH 5.5 Calculations Amount of acid component M=nV (1.8M)=(0.00055mol)(V) V=0.36mL Amount base component m=MM*n m=136.08g/mol*0.0043mol m=0..591g Actual measurement = 0.6143g Actual pH=5.46 Buffer 5 100 mL of 50mM Tris Buffer pH 7.5 Calculations Amount of Tris m=0.607g Actual mass m=0.6109g Titrate to pH 7.5 with 1.8M acetic acid Actual pH=7.55 Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.